ArticleACS sensors2021
Immobilization of Recombinant Fluorescent Biosensors Permits Imaging of Extracellular Ion Signals.
Article in ACS sensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 23 citations in OpenAlex.
- Imaging intercellular biomolecules by using fluorescent protein indicators with lipid-PEG anchors.Scientific reports · 2026Article
- Dry resist lamination for wafer-scale fabrication of microfluidic superfusion devices.Scientific reports · 2025Article
- mitoBKeLife · 2024Article
- Non-invasive single cell aptasensing in live cells and animals.Chemical science · 2024Article
- Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors.iScience · 2022Article
- A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.PLoS biology · 2022Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
Given the importance of ion gradients and fluxes in biology, monitoring ions locally at the exterior of the plasma membrane of intact cells in a noninvasive manner is highly desirable but challenging. Classical targeting of genetically encoded biosensors at the exterior of cell surfaces would be a suitable approach; however, it often leads to intracellular accumulation of the tools in vesicular structures and adverse modifications, possibly impairing sensor functionality. To tackle these issues, we generated recombinant fluorescent ion biosensors fused to traptavidin (TAv) specifically coupled to a biotinylated AviTag expressed on the outer cell surface of cells. We show that purified chimeras of TAv and pH-Lemon or GEPII 1.0, Förster resonance energy transfer-based pH and K
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.