ArticleDevelopment (Cambridge, England)2021
Integrated molecular analysis identifies a conserved pericyte gene signature in zebrafish.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 31 citations in OpenAlex.
- Endothelial Cell-Pericyte Crosstalk in the Brain Balances Microvascular Stability and Plasticity.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Hypoxia-activated scleraxis a mediates epicardial progenitor differentiation into a unique cardiac perivascular cell type.Nature communications · 2026Article
- Article
- Tumor-Associated Pericytes: Tumorigenicity and Targeting for Cancer Therapy.Current vascular pharmacology · 2026Review
- Cd248a regulates pericyte development and viability in zebrafish.Communications biology · 2025Article
- Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries.European journal of cell biology · 2025Article
- Review
- Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries.bioRxiv : the preprint server for biology · 2024Article
- Identification of distinct vascular mural cell populations during zebrafish embryonic development.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Distinct features of the regenerating heart uncovered through comparative single-cell profiling.Biology open · 2024Article
- The development of brain pericytes requires expression of the transcription factor nkx3.1 in intermediate precursors.PLoS biology · 2024Article
- Pericytes in the disease spotlight.Trends in cell biology · 2024Review
- Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development.Developmental cell · 2023Article
- Single-cell analysis reveals distinct fibroblast plasticity during tenocyte regeneration in zebrafish.Science advances · 2023Article
- Defining the cellular complexity of the zebrafish bipotential gonad.Biology of reproduction · 2023Article
- Biology of vascular mural cells.Development (Cambridge, England) · 2023Review
- Development and repair of blood vessels in the zebrafish spinal cord.Open biology · 2023Article
- Distinct features of the regenerating heart uncovered through comparative single-cell profiling.bioRxiv : the preprint server for biology · 2023Article
- Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development.bioRxiv : the preprint server for biology · 2023Article
- Identification of overlapping and distinct mural cell populations during early embryonic development.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Pericytes reside in capillary beds where they share a basement membrane with endothelial cells and regulate their function. However, little is known about embryonic pericyte development, in part, due to lack of specific molecular markers and genetic tools. Here, we applied single cell RNA-sequencing (scRNA-seq) of platelet derived growth factor beta (pdgfrb)-positive cells to molecularly characterize pericytes in zebrafish larvae. scRNA-seq revealed zebrafish cells expressing mouse pericyte gene orthologs, and comparison with bulk RNA-seq from wild-type and pdgfrb mutant larvae further refined a pericyte gene set. Subsequent integration with mouse pericyte scRNA-seq profiles revealed a core set of conserved pericyte genes. Using transgenic reporter lines, we validated pericyte expression of two genes identified in our analysis: NDUFA4 mitochondrial complex associated like 2a (ndufa4l2a), and potassium voltage-gated channel, Isk-related family, member 4 (kcne4). Both reporter lines exhibited pericyte expression in multiple anatomical locations, and kcne4 was also detected in a subset of vascular smooth muscle cells. Thus, our integrated molecular analysis revealed a molecular profile for zebrafish pericytes and allowed us to develop new tools to observe these cells in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.