ArticleFrontiers in molecular biosciences2021
Biomolecular Perturbations in In-Cell Dynamic Nuclear Polarization Experiments.
Article in Frontiers in molecular biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- High-frequency dynamic nuclear polarization in rotating solids.Science advances · 2026Review
- Bimodal Cholesterol for Correlative In-Cell DNP Solid-State NMR and Confocal Microscopy of the Plasma Membrane.Journal of the American Chemical Society · 2026Article
- Paramagnetic Agents for SE DNP: Synthesis and ESR Characterization of New Lipophilic Derivatives of Finland Trityl.Molecules (Basel, Switzerland) · 2025Article
- Pushing Sensitivity and Specificity Limits in Native Structural Biology:Journal of the American Chemical Society · 2025Article
- Systematic Evaluation of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR.Angewandte Chemie (International ed. in English) · 2025Article
- Topological Heterogeneity of Protein Kinase C Modulators in Human T-Cells Resolved with In-Cell Dynamic Nuclear Polarization NMR Spectroscopy.Journal of the American Chemical Society · 2024Article
- Expanding the tool box for native structural biology:Science advances · 2024Article
- Polarizing agents for efficient high field DNP solid-state NMR spectroscopy under magic-angle spinning: from design principles to formulation strategies.Chemical science · 2023Review
- The membrane activity of the antimicrobial peptide caerin 1.1 is pH dependent.Biophysical journal · 2023Article
- In-Cell Quantification of Drugs by Magic-Angle Spinning Dynamic Nuclear Polarization NMR.Journal of the American Chemical Society · 2022Article
- Solid-state NMR analysis of unlabeled fungal cell walls fromJournal of structural biology: X · 2022Article
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting forMagnetic resonance (Gottingen, Germany) · 2022Article
- In-Cell NMR of Intact Mammalian Cells Preserved with the Cryoprotectants DMSO and Glycerol Have Similar DNP Performance.Frontiers in molecular biosciences · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In-cell DNP is a growing application of NMR to the study of biomolecular structure and function within intact cells. An important unresolved question for in-cell DNP spectroscopy is the integrity of cellular samples under the cryogenic conditions of DNP. Despite the rich literature around cryopreservation of cells in the fields of stem cell/embryonic cell therapeutics, cell line preservation and in cryo-EM applications, the effect of cryopreservation procedures on DNP parameters is unclear. In this report we investigate cell survival and apoptosis in the presence of cryopreserving agents and DNP radicals. We also assess the effects of these reagents on cellular enhancements. We show that the DNP radical AMUPol has no effect on membrane permeability and does not induce apoptosis. Furthermore, the standard aqueous glass forming reagent, comprised of 60/30/10 d
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