ArticleChembiochem : a European journal of chemical biology2022
Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems.
Article in Chembiochem : a European journal of chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Novel tools for genomic modification and heterologous gene expression in the phylum Planctomycetota.Applied microbiology and biotechnology · 2025Article
- Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye.Angewandte Chemie (International ed. in English) · 2025Article
- Discovery of a Novel Photocaged PI3K Inhibitor Capable of Real-Time Reporting of Drug Release.ACS medicinal chemistry letters · 2023Article
- Light-Activated Translation of Different mRNAs in Cells via Wavelength-Dependent Photouncaging.Angewandte Chemie (International ed. in English) · 2023Article
- Recent Synthetic Biology Approaches for Temperature- and Light-Controlled Gene Expression in Bacterial Hosts.Molecules (Basel, Switzerland) · 2022Review
- Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems.Chembiochem : a European journal of chemical biology · 2022Article
- Light-mediated control of gene expression in the anoxygenic phototrophic bacteriumFrontiers in bioengineering and biotechnology · 2022Article
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Authors and funding
10 authors.
Funding
Abstract
Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically characterized, with the absorption exhibiting a red-shift. Their differing linkage through ether, carbonate, and carbamate bonds revealed that carbonate and carbamate bonds are convenient. Subsequently, those compounds were successfully applied in vivo for controlling gene expression in E. coli via blue light illumination. Furthermore, benzoate-based expression systems were subjected to light control by establishing a novel photocaged salicylic acid derivative. Besides its synthesis and in vitro characterization, we demonstrate the challenging choice of a suitable promoter system for light-controlled gene expression in E. coli. We illustrate various bottlenecks during both photocaged inducer synthesis and in vivo application and possibilities to overcome them. These findings pave the way towards novel caged inducer-dependent systems for wavelength-selective gene expression.
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Registered trials
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