Evidence map›Paper›PMID 34750572›Full record

ReviewNature biotechnology2021

Nanopore sequencing technology, bioinformatics and applications.

Yunhao Wang, Yue Zhao, Audrey Bollas, Yuru Wang, Kin Fai Au

Abstract readReview
In one paragraph

Review in Nature biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 953 papers.

0numbers the graph read from it
0cells of the map it votes in
953citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

953 citing papers in PubMed.

  1. Nanopore Sequencing for Chikungunya Virus: Principles and Application.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Review
  3. Review
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  6. Review
  7. Article
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  12. De novo design of semisynthetic conduction pores.Nature structural & molecular biology · 2026
    Article
  13. Review
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article

893 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yunhao Wang *Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.
Yue Zhao *Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.
Audrey Bollas *Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.
Yuru WangDepartment of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.
Kin Fai AuDepartment of Biomedical Informatics, The Ohio State University, Columbus, OH, USA. kinfai.au@osumc.edu.ORCID http://orcid.org/0000-0002-9222-4241

Funding

Quantitative and function analysis platform for repetitive genes and gene isoforms in pluripotency regulation and differentiationsR01HG011469 · NHGRI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI AU, KIN FAI · 2021 to 2024
$2.6M
Bioinformatics platform for Hybrid-Seq transcriptome data analysisR01HG008759 · NHGRI · UNIVERSITY OF IOWA · PI AU, KIN FAI · 2016 to 2020
$1.9M
Experimental and bioinformatics platform for epigenome analysis using nanopore sequencingR01GM136886 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI AU, KIN FAI · 2021 to 2024
$1.7M
NHGRI NIH HHS R01 HG008759NHGRI NIH HHS R01 HG011469NIGMS NIH HHS R01 GM136886
6 · The paper itself

Abstract

Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for investigations of genomes, transcriptomes, epigenomes and epitranscriptomes. Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the development of new nanopores, base-calling methods and experimental protocols tailored to particular applications.

Indexed as

NanoporesNanopore SequencingComputational BiologyHigh-Throughput Nucleotide SequencingSequence Analysis, DNATechnology

Identifiers

PMID34750572
PMCPMC8988251

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.