ArticleScientific reports2021
Infrared-spectroscopic, dynamic near-field microscopy of living cells and nanoparticles in water.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Infrared Near-Field Microscopy as a Platform for Nanoscale Biomedical Spectroscopy and Imaging.ACS applied materials & interfaces · 2026Review
- Infrared Nano-Spectroscopy of Single Cell Sections at the Ultrastructural Level.Nanophotonics (Berlin, Germany) · 2026Article
- Infrared nanoscopy for subcellular chemical imaging.QRB discovery · 2026Review
- Nano-Infrared Imaging and Spectroscopy of Animal Cells in Liquid Environment.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Transient infrared nanoscopy resolves the millisecond photoswitching dynamics of single lipid vesicles in water.Nature communications · 2025Article
- Directional Coupling to a λ/5000 Nanowaveguide.ACS nano · 2024Article
- Nanoscale Mechanical Manipulation of Ultrathin SiN Membranes Enabling Infrared Near-Field Microscopy of Liquid-Immersed samples.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Scanning Probe Nano-Infrared Imaging and Spectroscopy of Biochemical and Natural Materials.Small science · 2024Article
- Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale.Scientific reports · 2024Article
- Article
- In vitro investigation of protein assembly by combined microscopy and infrared spectroscopy at the nanometer scale.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Scattering-type Scanning Near-Field Optical Microscopy of Polymer-Coated Gold Nanoparticles.ACS omega · 2022Article
- High-fidelity nano-FTIR spectroscopy by on-pixel normalization of signal harmonics.Nanophotonics (Berlin, Germany) · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infrared fingerprint spectra can reveal the chemical nature of materials down to 20-nm detail, far below the diffraction limit, when probed by scattering-type scanning near-field optical microscopy (s-SNOM). But this was impossible with living cells or aqueous processes as in corrosion, due to water-related absorption and tip contamination. Here, we demonstrate infrared s-SNOM of water-suspended objects by probing them through a 10-nm thick SiN membrane. This separator stretches freely over up to 250 µm, providing an upper, stable surface to the scanning tip, while its lower surface is in contact with the liquid and localises adhering objects. We present its proof-of-principle applicability in biology by observing simply drop-casted, living E. coli in nutrient medium, as well as living A549 cancer cells, as they divide, move and develop rich sub-cellular morphology and adhesion patterns, at 150 nm resolution. Their infrared spectra reveal the local abundances of water, proteins, and lipids within a depth of ca. 100 nm below the SiN membrane, as we verify by analysing well-defined, suspended polymer spheres and through model calculations. SiN-membrane based s-SNOM thus establishes a novel tool of live cell nano-imaging that returns structure, dynamics and chemical composition. This method should benefit the nanoscale analysis of any aqueous system, from physics to medicine.
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