ArticleScientific reports2021
Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I.
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Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 23 citations in OpenAlex.
- High-resolution structure of monomeric photosystem I from Thermosynechococcus vestitus.Photosynthesis research · 2026Article
- Structural Conservation of the AACS omega · 2026Article
- Structural study of monomeric and dimeric photosystem I-LHCI supercomplexes from a bryophyte.Communications biology · 2026Article
- Detergent Choice Shapes the Solution Structures of Photosystems I and II: Implications for Crystallization and High-Resolution Studies.The journal of physical chemistry. B · 2025Article
- Article
- Development of the Nanobeam X-ray Experiments instrument at PAL-XFEL.Journal of synchrotron radiation · 2025Article
- Development of a TSR-based method for understanding structural relationships of cofactors and local environments in photosystem I.BMC bioinformatics · 2025Article
- Real-Time XFEL Data Analysis at SLAC and NERSC: a Trial Run of Nascent Exascale Experimental Data Analysis.Concurrency and computation : practice & experience · 2024Article
- Impact of Peripheral Hydrogen Bond on Electronic Properties of the Primary Acceptor Chlorophyll in the Reaction Center of Photosystem I.International journal of molecular sciences · 2024Article
- Characterization of Biological Samples Using Ultra-Short and Ultra-Bright XFEL Pulses.Advances in experimental medicine and biology · 2024Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Torsion angles to map and visualize the conformational space of a protein.Protein science : a publication of the Protein Society · 2023Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Primary donor triplet states of Photosystem I and II studied by Q-band pulse ENDOR spectroscopy.Photosynthesis research · 2022Article
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Authors and funding
47 authors at 9 institutions in 6 countries.
Stephen M Keable *Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Adrian Kölsch *Institut für Biologie, Humboldt-Universität Zu Berlin, 10115, Berlin, Germany.
Philipp S Simon *Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Medhanjali DasguptaMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Ruchira ChatterjeeMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Senthil Kumar SubramanianInstitut für Biologie, Humboldt-Universität Zu Berlin, 10115, Berlin, Germany.
Rana HusseinInstitut für Biologie, Humboldt-Universität Zu Berlin, 10115, Berlin, Germany.
Mohamed IbrahimInstitut für Biologie, Humboldt-Universität Zu Berlin, 10115, Berlin, Germany.
In-Sik KimMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Isabel BogaczMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Hiroki MakitaMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Cindy C PhamMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Franklin D FullerLCLS, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.
Sheraz GulMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Daniel PaleyMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Louise LassalleMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Kyle D SutherlinMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Asmit BhowmickMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Nigel W MoriartyMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Iris D YoungMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Johannes P BlaschkeNational Energy Research Scientific Computing Center, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Casper de LichtenbergDepartment of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, 75237, Uppsala, Sweden.
Petko ChernevDepartment of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, 75237, Uppsala, Sweden.
Mun Hon CheahDepartment of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, 75237, Uppsala, Sweden.
Sehan ParkPohang Accelerator Laboratory, POSTECH, Pohang, 37673, Korea.
Gisu ParkPohang Accelerator Laboratory, POSTECH, Pohang, 37673, Korea.
Jangwoo KimPohang Accelerator Laboratory, POSTECH, Pohang, 37673, Korea.
Sang Jae LeePohang Accelerator Laboratory, POSTECH, Pohang, 37673, Korea.
Jaehyun ParkPohang Accelerator Laboratory, POSTECH, Pohang, 37673, Korea.
Kensuke TonoJapan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5198, Japan.
Shigeki OwadaJapan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5198, Japan.
Mark S HunterLCLS, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.
Alexander BatyukLCLS, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.
Roland OggenfussPaul Scherrer Institut, 5232, Villigen, Switzerland.
Mathias SanderPaul Scherrer Institut, 5232, Villigen, Switzerland.
Serhane ZerdanePaul Scherrer Institut, 5232, Villigen, Switzerland.
Dmitry OzerovPaul Scherrer Institut, 5232, Villigen, Switzerland.
Karol NassPaul Scherrer Institut, 5232, Villigen, Switzerland.
Henrik LemkePaul Scherrer Institut, 5232, Villigen, Switzerland.
Roman MankowskyPaul Scherrer Institut, 5232, Villigen, Switzerland.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Johannes MessingerDepartment of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, 75237, Uppsala, Sweden.
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Vittal K YachandraMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Junko YanoMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Athina ZouniInstitut für Biologie, Humboldt-Universität Zu Berlin, 10115, Berlin, Germany.
Jan KernMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. jfkern@lbl.gov.
Lawrence Berkeley National Laboratory · USPaul Scherrer Institute · CHHumboldt-Universität zu Berlin · DEPohang Accelerator LaboratoryUppsala University · SESLAC National Accelerator Laboratory · USJapan Synchrotron Radiation Research Institute · JPPohang University of Science and Technology · KRSPring-8 · JP
Funding
Taking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MDIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MHigh throughput sample delivery method for time resolved studies of enzyme reactions with X-ray and complementary techniquesR01GM126289 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI KERN, JAN F · 2017 to 2025
$4.6MStructure and Function of the Mn Oxygen-Evolving ComplexR56GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 2009 to 2009
$530kNIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R01 GM117126NIGMS NIH HHS R01 GM126289NIGMS NIH HHS R56 GM055302
Abstract
Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We have determined the structure of cyanobacterial PS I at room temperature (RT) using femtosecond X-ray pulses from an X-ray free electron laser (XFEL) that shows a clear expansion of the entire protein complex in the direction of the membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken at multiple XFEL beamlines. In the RT structure of PS I, we also observe conformational differences between the two branches in the reaction center around the secondary electron acceptors A
Indexed as
Crystallography, X-RayPhotosynthesisPhotosystem I Protein ComplexProtein Structure, TertiaryTemperatureThermosynechococcusVitamin K 1Photosystem I Protein ComplexVitamin K 1
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