Evidence map›Paper›PMID 34749732›Full record

ArticleDiagnostic pathology2021

Establishment of multiplex RT-PCR to detect fusion genes for the diagnosis of Ewing sarcoma.

Hitomi Ueno-Yokohata, Hajime Okita, Keiko Nakasato, Chikako Kiyotani, Motohiro Kato, Kimikazu Matsumoto, Nobutaka Kiyokawa, Atsuko Nakazawa, Takako Yoshioka

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Article
  3. Molecular testing of soft tissue tumors.Diagnostic cytopathology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hitomi Ueno-YokohataDepartment of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan. ueno-ht@ncchd.go.jp.
Hajime OkitaDepartment of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan. okita-h@keio.jp.
Keiko NakasatoDepartment of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
Chikako KiyotaniChildren's Cancer Center, National Center for Child Health and Development, Tokyo, 157-8535, Japan.
Motohiro KatoChildren's Cancer Center, National Center for Child Health and Development, Tokyo, 157-8535, Japan.
Kimikazu MatsumotoChildren's Cancer Center, National Center for Child Health and Development, Tokyo, 157-8535, Japan.
Nobutaka KiyokawaDepartment of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
Atsuko NakazawaDepartment of Clinical Research, Saitama Children's Medical Center, Saitama, Saitama, 330-8777, Japan.
Takako YoshiokaDepartment of Pathology, National Center for Child Health and Development, Tokyo, 157-8535, Japan.
National Center For Child Health and Development · JPSaitama Children's Medical Center · JPThe University of Tokyo · JP

Funding

grant from the national center for child health and development 2019-C3grant from the national center for child health and development 30E-2japan agency for medical research and development JP19ck0106333
6 · The paper itself

Abstract

backgroundDetection of the tumor-specific EWSR1/FUS-ETS fusion gene is essential to diagnose Ewing sarcoma. Reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization are commonly used to detect the fusion gene, and assays using next-generation sequencing have recently been reported. However, at least 28 fusion transcript variants have been reported, making rapid and accurate detection difficult.

methodsWe constructed two sets of multiplex PCR assays and evaluated their utility using cell lines and clinical samples.

resultsEWSR1/FUS-ETS was detected in five of six tumors by the first set, and in all six tumors by the second set. The fusion gene detected only by the latter was EWSR1-ERG, which completely lacked exon 7 of EWSR1. The fusion had a short N-terminal region of EWSR1 and showed pathologically atypical features.

conclusionsWe developed multiplex RT-PCR assays to detect EWSR1-ETS and FUS-ETS simultaneously. These assays will aid the rapid and accurate diagnosis of Ewing sarcoma. In addition, variants of EWSR1/FUS-ETS with a short N-terminal region that may have been previously missed can be easily detected.

Indexed as

FemaleHumansMaleMultiplex Polymerase Chain ReactionOncogene FusionProto-Oncogene Proteins c-etsRNA-Binding Protein EWSRNA-Binding Protein FUSSarcoma, EwingEWSR1 protein, humanFUS protein, humanProto-Oncogene Proteins c-etsRNA-Binding Protein EWSRNA-Binding Protein FUSBreakpointEwing sarcomaEWSR1Fusion geneGenetic diagnosisMultiplex RT–PCRTranscription factor

Identifiers

PMID34749732
PMCPMC8573982
OpenAlexW3214339365

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.