ArticlePLoS genetics2021
High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 43 citations in OpenAlex.
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- Dual Inhibition of MYRF Cleavage by Its JM Region and PAN-1 CCT Gates Developmental Timing inbioRxiv : the preprint server for biology · 2026Article
- A single-copy knock-in system: one plasmid to target all chromosomes in C. elegans.G3 (Bethesda, Md.) · 2025Article
- Heat shock proteins function as signaling molecules to mediate neuron-glia communication in C. elegans during aging.Nature neuroscience · 2025Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- Advances in CRISPR-Cas9 in lineage tracing of model animals.Animal models and experimental medicine · 2025Review
- Intracellular protein-lipid interactions drive presynaptic assembly prior to neurexin recruitment.Neuron · 2025Article
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.The Journal of cell biology · 2025Article
- Article
- Diffusion within the synaptonemal complex can account for signal transduction along meiotic chromosomes.Molecular biology of the cell · 2024Article
- A role for the C. elegans Argonaute protein CSR-1 in small nuclear RNA 3' processing.PLoS genetics · 2024Article
- Heat Shock Proteins Function as Signaling Molecules to Mediate Neuron-Glia Communication During Aging.bioRxiv : the preprint server for biology · 2024Article
- Development of aging research inCurrent research in food science · 2024Review
- Expansion of the split hygromycin toolkit for transgene insertion inmicroPublication biology · 2024Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Syndapin and GTPase RAP-1 control endocytic recycling via RHO-1 and non-muscle myosin II.Current biology : CB · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans strains that constitutively express Cas9 in the germline from an integrated transgene. These strains markedly improve the success rate for plasmid-based CRISPR edits. For simple, short homology arm GFP insertions, 50-100% of injected animals typically produce edited progeny, depending on the target locus. Template-guided editing from an extrachromosomal array is maintained over multiple generations. We have built strains with the Cas9 transgene on multiple chromosomes. Additionally, each Cas9 locus also contains a heatshock-driven Cre recombinase for selectable marker removal and a bright fluorescence marker for easy outcrossing. These integrated Cas9 strains greatly reduce the workload for producing individual genome edits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.