Evidence map›Paper›PMID 34746865›Full record

ArticleSTAR protocols2021

MegaGate: A toxin-less gateway molecular cloning tool.

Christian Kramme, Alexandru M Plesa, Helen H Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E Kohman, Pranam Chatterjee, George M Church

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Induction of Meiosis from Human Pluripotent Stem Cells.bioRxiv : the preprint server for biology · 2024
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Christian KrammeDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Alexandru M PlesaDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Helen H WangDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Bennett WolfDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Merrick Pierson SmelaDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Xiaoge GuoDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Richie E KohmanDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Pranam ChatterjeeDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
George M ChurchDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Harvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gateway cloning employs the use of the

Indexed as

Recombinant Fusion ProteinsCloning, MolecularDNA, ComplementaryEscherichia coliGene LibraryPlasmidsPolymerase Chain ReactionDNA, ComplementaryRecombinant Fusion ProteinsHigh-Throughput ScreeningMolecular BiologyStem Cells

Identifiers

PMID34746865
PMCPMC8551244
OpenAlexW3210119219

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.