Evidence map›Paper›PMID 34746858›Full record

ArticleSTAR protocols2021

RHybridFinder: An R package to process immunopeptidomic data for putative hybrid peptide discovery.

Frederic Saab, David J Hamelin, Qing Ma, Kevin A Kovalchik, Isabelle Sirois, Pouya Faridi, Chen Li, Anthony W Purcell, Peter Kubiniok, Etienne Caron

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Frederic SaabCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
David J HamelinCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
Qing MaSchool of Electrical Engineering and Computer Science, Faculty of Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Kevin A KovalchikCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
Isabelle SiroisCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
Pouya FaridiInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Chen LiInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Anthony W PurcellInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Peter KubiniokCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
Etienne CaronCHU Sainte-Justine Research Center, Montreal, QC H3T 1C5, Canada.
Centre Hospitalier Universitaire Sainte-Justine · CAAustralian Regenerative Medicine Institute · AUUniversity of Ottawa · CA

Funding

CIHR 174924
6 · The paper itself

Abstract

Identification of proteasomal spliced peptides (PSPs) by mass spectrometry (MS) is not possible with traditional search engines. Here, we provide a protocol for running RHybridFinder (RHF), an R package for the computational inference of putative PSPs detected by MS. RHF extracts high confidence scored de novo sequenced peptides identified by PEAKS software. Those peptides are then matched to protein databases to infer cis- or trans-spliced major histocompatibility complex (MHC)-associated peptides. RHF is relatively fast and straightforward. PSPs have to be validated experimentally. For complete details on the use and execution of the original protocol, please refer to Faridi et al. (2018).

Indexed as

PeptidesProteasome Endopeptidase ComplexSoftwareDatabases, ProteinEpitopesHumansMass SpectrometryProteomicsEpitopesPeptidesProteasome Endopeptidase ComplexBioinformaticsCancerComputer sciencesImmunologyMass Spectrometry

Identifiers

PMID34746858
PMCPMC8551247
OpenAlexW3209241819

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.