Evidence map›Paper›PMID 34746759›Full record

ArticlePlant communications2021

Split-HaloTag imaging assay for sophisticated microscopy of protein-protein interactions

Rieke Minner-Meinen, Jan-Niklas Weber, Andreas Albrecht, Rainer Matis, Maria Behnecke, Cindy Tietge, Stefan Frank, Jutta Schulze, Henrik Buschmann, Peter Jomo Walla and 3 more

Abstract read
In one paragraph

Article in Plant communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025
    Review
  6. Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Rieke Minner-MeinenInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Jan-Niklas WeberInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Andreas AlbrechtInstitut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Hagenring 30.023c, 38106 Braunschweig, Germany.
Rainer MatisInstitut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Hagenring 30.023c, 38106 Braunschweig, Germany.
Maria BehneckeInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Cindy TietgeInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Stefan FrankInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Jutta SchulzeInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Henrik BuschmannBotany Department, Universität Osnabrück, Barbara Strasse 11, 49076 Osnabrück, Germany.
Peter Jomo WallaInstitut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Hagenring 30.023c, 38106 Braunschweig, Germany.
Ralf-R MendelInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
Robert HänschInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.
David KaufholdtInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, 38106 Braunschweig, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An ever-increasing number of intracellular multi-protein networks have been identified in plant cells. Split-GFP-based protein-protein interaction assays combine the advantages of

Indexed as

Protein Interaction Domains and MotifsBotanyPlantsadvanced microscopycytoskeletongateway cloningmolybdenum cofactor biosynthesis complexphotostable fluorescent dyesprotein–protein interactionSplit-HaloTag imaging assay

Identifiers

PMID34746759
PMCPMC8555439

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.