Evidence map›Paper›PMID 34746704›Full record

ArticleiScience2021

Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate.

Liang Guo, Kan Zhu, Michael Pargett, Adam Contreras, Patrick Tsai, Quan Qing, Wolfgang Losert, John Albeck, Min Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Liang GuoDepartment of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA.
Kan ZhuDepartment of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA.
Michael PargettDepartment of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA.
Adam ContrerasDepartment of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA.
Patrick TsaiDepartment of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA.
Quan QingDepartment of Physics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Wolfgang LosertDepartment of Physics, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.
John AlbeckDepartment of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA.
Min ZhaoDepartment of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA.
California Institute for Regenerative Medicine · USUniversity of California, Davis · USArizona State University · USHarbin Engineering University · CNUniversity of Maryland, College Park · US

Funding

VISION RESEARCH CORE GRANTP30EY012576 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI MARIE E BURNS · 1999 to 2026
$18.9M
Molecular Generators at Corneal Wounds Produce and regulate the Wound Electrical SignalsR01EY019101 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2010 to 2023
$5.2M
The role of dynamics in defining the limits of normal developmental signaling.R01GM115650 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ALBECK, JOHN G. · 2016 to 2021
$1.8M
NEI NIH HHS P30 EY012576NEI NIH HHS R01 EY019101NIGMS NIH HHS R01 GM115650
6 · The paper itself

Abstract

Intracellular signaling dynamics play fundamental roles in cell biology. Precise modulation of the amplitude, duration, and frequency of signaling activation will be a powerful approach to investigate molecular mechanisms as well as to engineer signaling to control cell behaviors. Here, we showed a practical approach to achieve precise amplitude modulation (AM), frequency modulation (FM), and duration modulation (DM) of MAP kinase activation. Alternating current (AC) electrical stimulation induced synchronized ERK activation. Amplitude and duration of ERK activation were controlled by varying stimulation strength and duration. ERK activation frequencies were arbitrarily modulated with trains of short AC applications with accurately defined intervals. Significantly, ERK dynamics coded by well-designed AC can rewire PC12 cell fate independent of growth factors. This technique can be used to synchronize and modulate ERK activation dynamics, thus would offer a practical way to control cell behaviors

Indexed as

BiotechnologyCell biologyFunctional aspects of cell biologyIntegrative aspects of cell biology

Identifiers

PMID34746704
PMCPMC8554532
OpenAlexW3203968956

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.