ArticleFrontiers in veterinary science2021
Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Sperm Recovery and Evaluation in Domestic Species: Challenges and Current Practices.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Clustering of sperm by chromatin integrity features reveals breed and monthly effects, as well as a link between double-strand DNA breaks and litter size in pigs.BMC veterinary research · 2026Article
- Large-scale transcriptomic analyses reveal downstream target genes of ZFY1 and ZFY2 transcription factors in male germ cells.Cell death and differentiation · 2026Article
- Effect of Sperm DNA Fragmentation and Chromatin Decondensation on PLCζ Efficacy in Infertile Patients.Current issues in molecular biology · 2025Article
- Comparative developmental competence ofVeterinary world · 2024Article
- Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.International journal of molecular sciences · 2023Article
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
- Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.Journal of animal science and biotechnology · 2022Article
- Sperm degradation after vasectomy follows a sperm chromatin fragmentation-dependent mechanism causing DNA breaks in the toroid linker regions.Molecular human reproduction · 2022Article
- Sperm selection with hyaluronic acid improved live birth outcomes among older couples and was connected to sperm DNA quality, potentially affecting all treatment outcomes.Human reproduction (Oxford, England) · 2022Article
- Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs.Biological research · 2022Article
- Is oxidative stress evaluated in viable human spermatozoa a marker of good semen quality?Frontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although sperm chromatin damage, understood as damage to DNA or affectations in sperm protamination, has been proposed as a biomarker for sperm quality in both humans and livestock, the low incidence found in some animals raises concerns about its potential value. In this context, as separate methods measure different facets of chromatin damage, their comparison is of vital importance. This work aims at analyzing eight techniques assessing chromatin damage in pig sperm. With this purpose, cryopreserved sperm samples from 16 boars were evaluated through the following assays: TUNEL, TUNEL with decondensation, SCSA, alkaline and neutral sperm chromatin dispersion (SCD) tests, alkaline and neutral Comet assays, and chromomycin A3 test (CMA3). In all cases, the extent of chromatin damage and the percentage of sperm with fragmented DNA were determined. The degree of chromatin damage and the percentage of sperm with fragmented DNA were significantly correlated (
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