Evidence map›Paper›PMID 34745749›Full record

ArticleBiomedical optics express2021

Improved microscopy with ultraviolet surface excitation (MUSE) using high-index immersion illumination.

Vincent D Ching-Roa, Chi Z Huang, Michael G Giacomelli

Abstract read
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In one paragraph

Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Vincent D Ching-RoaDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, NY 14627, USA.ORCID https://orcid.org/0000-0003-3836-534X
Chi Z HuangDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, NY 14627, USA.
Michael G GiacomelliDepartment of Biomedical Engineering, University of Rochester, 207 Goergen Hall, Box 270168, Rochester, NY 14627, USA.

Funding

Two photon fluorescence microscopy for dermatologic surgery and biopsyR37CA258376 · NCI · UNIVERSITY OF ROCHESTER · PI Michael Gene Giacomelli · 2021 to 2026
$2.0M
Fluorescence microscopy for evaluation of Mohs surgical marginsK22CA226035 · NCI · UNIVERSITY OF ROCHESTER · PI GIACOMELLI, MICHAEL GENE · 2019 to 2021
$559k
NCI NIH HHS K22 CA226035NCI NIH HHS R37 CA258376
6 · The paper itself

Abstract

Microscopy with ultraviolet surface excitation (MUSE) typically has an optical sectioning thickness significantly larger than standard physical sectioning thickness, resulting in increased background fluorescence and higher feature density compared to formalin-fixed, paraffin-embedded physical sections. We demonstrate that high-index immersion with angled illumination significantly reduces optical sectioning thickness through increased angle of refraction of excitation light at the tissue interface. We present a novel objective dipping cap and waveguide-based MUSE illuminator design with high-index immersion and quantify the improvement in optical sectioning thickness, demonstrating an e

Identifiers

PMID34745749
PMCPMC8547983

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.