Evidence map›Paper›PMID 34743273›Full record

ArticleMolecular biology reports2022

Screening and stability analysis of reference genes in fasting caecotrophy model in rabbits.

Hui He, Zhichao Li, Mengke Ni, Shanshan Xing, Lei Yu, Huifen Xu, Dehu Zhuo, Ming Li

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. International journal of molecular sciences · 2023
    Article
  2. Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Hui He *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China.
Zhichao Li *College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China.
Mengke NiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China.
Shanshan XingCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China.
Lei YuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China.
Huifen XuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China. huifen221@126.com.ORCID http://orcid.org/0000-0003-3963-2868
Dehu ZhuoCollege of Medicine, Zhengzhou University, Zhengzhou, 450046, People's Republic of China. zzuyxzdh@163.com.
Ming LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, People's Republic of China. 13803849306@163.com.
Henan Agricultural University · CNHenan University of Technology · CNZhengzhou University · CN

Funding

national key research and development program of china 2018YFD0502203
6 · The paper itself

Abstract

backgroundThe selection and validation of stably expressed reference genes is key for accurately quantifying the mRNA abundance of genes under different treatments. In the rabbit model of fasting caecotrophy, reports about the selection of stable reference genes are not available. METHODS AND

resultsThis study aims to screen suitable reference genes in different tissues (including uterus, cecum, and liver) of rabbits between control and fasting caecotrophy groups. RT-qPCR was used to analyze the expression levels of eight commonly used reference genes (including GAPDH, 18S rRNA, B2M, CYP, HPRT1, β-actin, H2afz, Ywhaz), and RefFinder (including geNorm, NormFinder, and BestKeeper) was used to analyze the expression stability of these reference genes. Our results showed that the most stable reference genes were different in different tissues and treatments. In the control and fasting caecotrophy groups, CYP, GAPDH and HPRT1 were proven to be the top stable reference genes in the uterus, cecum, and liver tissues, respectively. GAPDH and Ywhaz were proven to be the top two stable reference genes among uterus, cecum, and liver in both control and fasting caecotrophy groups.

conclusionsOur results indicated that the combined analysis of three or more reference genes (GAPDH, HPRT1, and Ywhaz) are recommended to be used for RT-qPCR normalization in the rabbit model of fasting caecotrophy, and that GAPDH is a better choice than the other reference genes for normalizing the relative expression of target genes in different tissues of fasting caecotrophy rabbits.

Indexed as

14-3-3 ProteinsAnimalsCoprophagiaFastingFecesFeeding BehaviorGene ExpressionGene Expression ProfilingGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)Hypoxanthine PhosphoribosyltransferaseLiverRabbitsReal-Time Polymerase Chain ReactionReference StandardsRNA, MessengerTranscriptome14-3-3 ProteinsGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)Hypoxanthine PhosphoribosyltransferaseRNA, MessengerCoprophagyExpression stabilityNew Zealand white rabbitReference genes

Identifiers

PMID34743273
OpenAlexW3212516867

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.