ArticleInternational journal of pharmaceutics2021
Bovine mammary alveolar MAC-T cells afford a tool for studies of bovine milk exosomes in drug delivery.
Article in International journal of pharmaceutics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Milk-Derived Extracellular Vesicles InhibitAnimals : an open access journal from MDPI · 2026Article
- Comprehensive multiomic analysis of extracellular vesicles from Mycoplasma bovis-infected bovine mammary epithelial cells identifies proteins and miRNAs that induce inflammatory responses in macrophages.Veterinary research · 2025Article
- From biomolecules to breakthroughs: exosomes as next-generation theranostics in female infertility.Frontiers in cell and developmental biology · 2025Review
- YTHDF2-mediated mFrontiers in cellular and infection microbiology · 2025Article
- Immunotherapy in mastitis: state of knowledge, research gaps and way forward.The veterinary quarterly · 2024Review
- The role of RNA epigenetic modification-related genes in the immune response of cattle to mastitis induced by Staphylococcus aureus.Animal bioscience · 2024Article
- Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells.Biological trace element research · 2024Article
- Milk-borne small extracellular vesicles: kinetics and mechanisms of transport, distribution, and elimination.Extracellular vesicles and circulating nucleic acids · 2023Article
- Regulation of adipogenesis by exosomal milk miRNA.Reviews in endocrine & metabolic disorders · 2023Review
- Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues.Animals : an open access journal from MDPI · 2022Review
- Exosomes in Mastitis-Research Status, Opportunities, and Challenges.Animals : an open access journal from MDPI · 2022Review
- Milk exosomes in nutrition and drug delivery.American journal of physiology. Cell physiology · 2022Review
- Assessing extracellular vesicles from bovine mammary gland epithelial cells cultured in FBS-free medium.Extracellular vesicles and circulating nucleic acids · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bovine milk exosomes (BMEs) have attracted attention as vehicles for delivering RNA therapeutics. BMEs originate in mammary alveolar cells. Here, we determined whether bovine mammary alveolar MAC-T cells afford a tool to assess RNA delivery by BMEs. MAC-T cells exosomes (MAC-T BMEs) and BMEs were harvested by differential ultracentrifugation. Exosome size, morphology, microRNA content and marker proteins were assessed using nanoparticle tracking analysis, transmission electron microscopy, real-time PCR and immunoblot analysis, respectively. MAC-T cells were genetically engineered to secrete MAC-T BMEs endogenously labeled with a near-infrared fluorescent protein and tissue distribution was compared to fluorophore-labeled BMEs following intravenous injection in C57BL/6 mice. Morphology and size were similar in MAC-T BMEs and BMEs (94 ± 5.8 nm and 101 ± 4.2 nm, p > 0.05). Both preparations expressed miR-320a, miR-200c and let-7a-5p (positive controls) but not miR-1 (negative control). Exosome marker proteins, CD9, CD63, CD81 and Tsg101, were detected in both MAC-T BMEs and BMEs. Distribution in mouse tissues was similar for both preparations, with liver being the primary accumulation site. Collectively, MAC-T BMEs afford a tool for BMEs-based RNA delivery studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.