Evidence map›Paper›PMID 34742256›Full record

ArticleBMC nephrology2021

LncRNA NNT-AS1 regulates proliferation, ECM accumulation and inflammation of human mesangial cells induced by high glucose through miR-214-5p/smad4.

Zhuang Geng, Xiang Wang, Shiyuan Hao, Bingzi Dong, Yajing Huang, Yangang Wang, Lili Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC nephrology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Long Non-Coding RNAs in the Pathogenesis of Diabetic Kidney Disease.Frontiers in cell and developmental biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Zhuang GengDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China.
Xiang WangDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China.
Shiyuan HaoDepartment of Anesthesiology of the Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China.
Bingzi DongDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China.
Yajing HuangDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China.
Yangang WangDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China. wangyg@qdu.edu.cn.
Lili XuDepartment of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P.R. China. qdfyxll@qdu.edu.cn.
Affiliated Hospital of Qingdao University · CNQingdao University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLncRNA NNT-AS1 (NNT-AS1) has been extensively studied as the causative agent in propagation and progression of lung and bladder cancers, and cholangiocarcinoma. However, its significance in proliferation and inflammation of diabetic nephropathy is enigmatic. This study focuses on the molecular mechanisms followed by NNT-AS1 to establish diabetic nephropathy (DN) and its potential miRNA target.

methodsBioinformatics analysis to identify potential miRNA target of NNT-AS1 and smad4 transcription factor was conducted using LncBase and TargetScan, and was subsequently confirmed by luciferase reporter assay. Relative quantitative expression of NNT-AS1 in human glomerular mesangial cells (HGMCs) was detected through quantitative real-time PCR and WB analysis. Cell proliferation was detected through CCK-8 assay, whereas, ELISA was conducted to evaluate the expression of inflammatory cytokines. Following this, relative expression of miR-214-5p and smad4 were confirmed through qRT-PCR and western blot analysis.

resultsResults from the experiments manifested up-regulated levels of NNT-AS1 and smad4 in the blood samples of DN patients as well as in HGMCs, whereas, downregulated levels of miR-214-5p were measured in the HGMCs suggesting the negative correlation between NNT-AS1 and miR-214-5p. Potential binding sites of NNT-AS1 showed miR-214-5p as its direct target and NNT-AS1 as potential absorber for this microRNA, in turn increasing the expression of transcription factor smad4.

conclusionThe data suggests that NNT-AS1 can be positively used as a potential biomarker and indicator of DN and causes extracellular matrix (ECM) accumulation and inflammation of human mesangial cells.

Indexed as

Cell ProliferationBlood GlucoseDiabetic NephropathiesDown-RegulationExtracellular MatrixHumansInflammationMesangial CellsMicroRNAsMitochondrial ProteinsNADP Transhydrogenase, AB-SpecificRNA, Long NoncodingSmad4 ProteinUp-RegulationBlood GlucoseMicroRNAsMIRN214 microRNA, humanMitochondrial ProteinsNADP Transhydrogenase, AB-SpecificNNT protein, humanRNA, Long NoncodingSmad4 ProteinSMAD4 protein, humanDiabetic nephropathyExtracellular matrixHuman mesangial cellsmiR-214-5pNNT-AS1smad4

Identifiers

PMID34742256
PMCPMC8572446
OpenAlexW3213005023

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.