ArticleAdvanced healthcare materials2022
Nucleic Acid Delivery from Granular Hydrogels.
Article in Advanced healthcare materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Biological Nanoscaffolds from Hierarchical Construction to Applications.Molecules (Basel, Switzerland) · 2026Review
- Granular Hydrogels as Modular Biomaterials: From Structural Design to Biological Responses.Advanced healthcare materials · 2026Review
- Immiscible Phase Separation-Driven Microfabrication of Gelatin Methacryloyl Scaffolds for BMP-2 Delivery and Osteogenic Enhancement.Macromolecular bioscience · 2025Article
- Practical Guide to the Design of Granular Hydrogels for Customizing Complex Cellular Microenvironments.Advanced healthcare materials · 2025Review
- Delivery of Nucleic Acids for Vascular Tissue Engineering Applications.Regenerative engineering and translational medicine · 2025Article
- Foreign Body Immune Response to Zwitterionic and Hyaluronic Acid Granular Hydrogels Made with Mechanical Fragmentation.Advanced healthcare materials · 2025Article
- Injectable Microporous Annealed Crescent-Shaped (MAC) Particle Hydrogel Scaffold for Enhanced Cell Infiltration.Advanced healthcare materials · 2024Article
- Granular Hydrogels for Harnessing the Immune Response.Advanced healthcare materials · 2024Review
- Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Advancing Engineered Plant Living Materials through Tobacco BY-2 Cell Growth and Transfection within Tailored Granular Hydrogel Scaffolds.ACS central science · 2024Article
- Gelatin methacryloyl granular scaffolds for localized mRNA delivery.Aggregate (Hoboken, N.J.) · 2024Article
- Review
- Macromolecular Polymer Based Complexes: A Diverse Strategy for the Delivery of Nucleotides.Protein and peptide letters · 2024Review
- Dynamically Cross-Linked Granular Hydrogels for 3D Printing and Therapeutic Delivery.ACS applied bio materials · 2023Article
- Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage.Science advances · 2023Article
- The novel hyaluronic acid granular hydrogel attenuates osteoarthritis progression by inhibiting the TLR-2/NF-κB signaling pathway through suppressing cellular senescence.Bioengineering & translational medicine · 2023Article
- Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture.Journal of visualized experiments : JoVE · 2022Article
- Recent Advances in Macroporous Hydrogels for Cell Behavior and Tissue Engineering.Gels (Basel, Switzerland) · 2022Review
- Particle fraction is a bioactive cue in granular scaffolds.Acta biomaterialia · 2022Article
- 4D Printing of Extrudable and Degradable Poly(Ethylene Glycol) Microgel Scaffolds for Multidimensional Cell Culture.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nucleic acid delivery has applications ranging from tissue engineering to vaccine development to infectious disease. Cationic polymer condensed nucleic acids are used with surface-coated porous scaffolds and are able to promote long-term gene expression. However, due to surface loading of the scaffold, there is a limit to the amount of nucleic acid that can be loaded, resulting in decreasing expression rate over time. In addition, surface-coated scaffolds are generally non-injectable. Here, it is demonstrated that cationic polymer condensed nucleic acids can be effectively loaded into injectable granular hydrogel scaffolds by stabilizing the condensed nucleic acid into a lyophilized powder, loading the powder into a bulk hydrogel, and then fragmenting the loaded hydrogel. The resulting hydrogel microparticles contain non-aggregated nucleic acid particles, can be annealed post-injection to result in an injectable microporous hydrogel, and can effectively deliver nucleic acids to embedded cells with a constant expression rate. Due to the nature of granular hydrogels, it is demonstrated that mixtures of loaded and unloaded particles and spatially resolved gene expression can be easily achieved. The ability to express genes long term from an injectable porous hydrogel will further open the applications of nucleic acid delivery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.