Evidence map›Paper›PMID 34741801›Full record

ArticleCell2021

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes.

Suresh Kumar, Ruheena Javed, Michal Mudd, Sandeep Pallikkuth, Keith A Lidke, Ashish Jain, Karthikeyan Tangavelou, Sigurdur Runar Gudmundsson, Chunyan Ye, Tor Erik Rusten and 12 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
11.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 103 citations in OpenAlex.

  1. Article
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  8. ATG16L1 controls mammalian vacuolar proton ATPase.The Journal of cell biology · 2025
    Article
  9. A role for CASM in the repair of damaged Golgi architecture.bioRxiv : the preprint server for biology · 2025
    Article
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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 6 institutions in 4 countries.

Suresh KumarAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Ruheena JavedAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Michal MuddAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Sandeep PallikkuthDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.
Keith A LidkeDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.
Ashish JainFaculty of Medicine, University of Oslo, Oslo, Norway.
Karthikeyan TangavelouAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Sigurdur Runar GudmundssonUniversity of Iceland, Reykjavík, Iceland.
Chunyan YeCenter for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Tor Erik RustenFaculty of Medicine, University of Oslo, Oslo, Norway.
Jan Haug AnonsenInstitute of Biosciences, University of Oslo, Oslo, Norway.
Alf Håkon LystadFaculty of Medicine, University of Oslo, Oslo, Norway.
Aurore Claude-TaupinAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Anne SimonsenFaculty of Medicine, University of Oslo, Oslo, Norway.
Michelle SalemiProteomics Core, University of California Davis, Davis, CA, USA.
Brett PhinneyProteomics Core, University of California Davis, Davis, CA, USA.
Jing LiDepartment of Surgery, University of Virginia, Charlottesville, VA, USA.
Lian-Wang GuoDepartment of Surgery, University of Virginia, Charlottesville, VA, USA.
Steven B BradfuteCenter for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Graham S TimminsAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; School of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
Eeva-Liisa EskelinenInstitute of Biomedicine, University of Turku, Turku, Finland.
Vojo DereticAutophagy Inflammation and Metabolism Center, University of New Mexico Health Sciences Center, Albuquerque, NM, USA; Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA. Electronic address: vderetic@salud.unm.edu.
University of New Mexico · USUniversity of Oslo · NOUniversity of California, Davis · USUniversity of Virginia · USUniversity of Iceland · ISUniversity of Turku · FI

Funding

Unfolded Protein Response and Autophagy in T Helper Cell Effector FunctionP20GM121176 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI VOJO P DERETIC · 2017 to 2026
$24.9M
Autophagy Against Tuberculosis and HIVR01AI111935 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI DERETIC, VOJO P · 2014 to 2023
$7.0M
Pattern Recognition Receptors and Autophagy in Mtb Control in AIDSR37AI042999 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI VOJO P DERETIC · 2018 to 2026
$4.7M
BET Bromodomain proteins as Novel Epigenetic Targets for prevention of Intimal Hyperplasia after Vascular SurgeryR01HL133665 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GUO, LIANWANG · 2016 to 2019
$1.6M
Master epigenetic regulators and retinal degenerative diseaseR01EY029809 · NEI · UNIVERSITY OF VIRGINIA · PI GUO, LIANWANG · 2019 to 2022
$1.6M
Phinney - Orbitrap Fusion ETDS10OD021801 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2016 to 2016
$600k
QE-HF-X Mass SpectrometerS10OD026918 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2020 to 2020
$600k
NEI NIH HHS R01 EY029809NHLBI NIH HHS R01 HL133665NIAID NIH HHS R01 AI111935NIAID NIH HHS R37 AI042999NIGMS NIH HHS P20 GM121176NIH HHS S10 OD021801NIH HHS S10 OD026918
6 · The paper itself

Abstract

The biogenesis of mammalian autophagosomes remains to be fully defined. Here, we used cellular and in vitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment. The autophagic precursors emerge through fusion of FIP200 vesicles, derived from the cis-Golgi, with endosomally derived ATG16L1 membranes to generate a hybrid pre-autophagosomal structure, HyPAS. A previously unrecognized apparatus defined here controls HyPAS biogenesis and mammalian autophagosomal precursor membranes. HyPAS can be modulated by pharmacological agents whereas its formation is inhibited upon severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or by expression of SARS-CoV-2 nsp6. These findings reveal the origin of mammalian autophagosomal membranes, which emerge via convergence of secretory and endosomal pathways, and show that this process is targeted by microbial factors such as coronaviral membrane-modulating proteins.

Indexed as

AutophagosomesAutophagyCell Line, TumorCOVID-19CRISPR-Cas SystemsEndoplasmic ReticulumEndosomesGolgi ApparatusHEK293 CellsHeLa CellsHumansMembrane FusionMicroscopy, ConfocalPhagosomesQa-SNARE ProteinsReceptors, sigmaATP2A2 protein, humanESYT2 protein, humanQa-SNARE ProteinsReceptors, sigmaSarcoplasmic Reticulum Calcium-Transporting ATPasesSigma-1 ReceptorSTX17 protein, humanSynaptotagminsATG16L1Atg8ylationautophagycoronavirusCOVID-19endosomeFIP200GolgiSARS-CoV-2Syntaxin 17

Identifiers

PMID34741801
PMCPMC8616855
OpenAlexW3213488744

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.