Evidence map›Paper›PMID 34741769›Full record

ArticleEquine veterinary journal2022

Extracellular vesicles from equine mesenchymal stem cells decrease inflammation markers in chondrocytes in vitro.

Magdalena Arévalo-Turrubiarte, Mario Baratta, Giovanna Ponti, Elisabetta Chiaradia, Eugenio Martignani

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Equine veterinary journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Magdalena Arévalo-TurrubiarteDepartment of Veterinary Science, University of Turin, Turin, Italy.
Mario BarattaDepartment of Veterinary Science, University of Turin, Turin, Italy.
Giovanna PontiDepartment of Veterinary Science, University of Turin, Turin, Italy.
Elisabetta ChiaradiaDepartment of Veterinary Medicine, University of Perugia, Perugia, Italy.
Eugenio MartignaniDepartment of Veterinary Science, University of Turin, Turin, Italy.ORCID https://orcid.org/0000-0001-7748-1832
University of Turin · ITUniversity of Parma · ITUniversity of Perugia · IT

Funding

Università degli Studi di Torino
6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) have been used therapeutically in equine medicine. MSCs release extracellular vesicles (EVs), which affect cell processes by inhibiting cell apoptosis and regulating inflammation. To date, little is known about equine EVs and their regenerative properties.

objectivesTo characterise equine MSC-derived extracellular vesicles (EVs) and evaluate their effect on equine chondrocytes treated with pro-inflammatory cytokines in vitro. STUDY

designIn vitro experiments with randomised complete block design.

methodsMesenchymal stem cells from bone marrow, adipose tissue, and synovial fluid were cultured in vitro. The MSC culture medium was centrifuged and filtered. Isolated particles were analysed for size and concentration (total number of particles per mL). Transmission electron microscopy analysis was performed to evaluate the morphology and CD9 expression of the particles. Chondrocytes from healthy equines were treated with the inflammatory cytokines interleukin (IL)-1β and tumour necrosis factor-alpha. MSC-derived EVs from bone marrow and synovial fluid cells were added as co-treatments in vitro. Gene expression analysis by real-time PCR was performed to evaluate the effects of EVs.

resultsThe particles isolated from MSCs derived from different tissues did not differ significantly in size and concentration. The particles had a round-like shape and positively expressed CD9. EVs from bone marrow cells displayed reduced expression of metalloproteinase-13. MAIN LIMITATIONS: Sample size and characterisation of the content of EVs.

conclusionsEVs isolated from equine bone marrow MSCs reduced metalloproteinase 13 gene expression; this gene encodes an enzyme related to cartilage degradation in inflamed chondrocytes in vitro. EVs derived from MSCs can reduce inflammation and could potentially be used as an adjuvant treatment to improve tissue and cartilage repair in the articular pathologies.

Indexed as

Extracellular VesiclesHorse DiseasesMesenchymal Stem CellsAnimalsChondrocytesCytokinesHorsesInflammationTumor Necrosis Factor-alphaCytokinesTumor Necrosis Factor-alphachondrocytesextracellular vesicleshorsemesenchymal stem cellspro-inflammation

Identifiers

PMID34741769
PMCPMC9787580
OpenAlexW3212592913

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.