Evidence map›Paper›PMID 34741352›Full record

ArticleJournal of clinical laboratory analysis2021

A rapid lateral flow immunoassay strip for detection of SARS-CoV-2 antigen using latex microspheres.

Lin Shen, Qihan Zhang, Xiaolu Luo, Haolin Xiao, Miao Gu, Liangli Cao, Feijun Zhao, Zhencheng Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Lin ShenSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.ORCID https://orcid.org/0000-0003-1462-276X
Qihan ZhangSchool of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin, China.
Xiaolu LuoClinical Laboratory, The Fourth People's Hospital of Nanning, Nanning, China.
Haolin XiaoSchool of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin, China.
Miao GuSchool of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.
Liangli CaoSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.
Feijun ZhaoSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.
Zhencheng ChenSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.
Guilin University of Electronic Technology · CNThe Fourth People's Hospital · CN

Funding

Guangxi Key Reasearch and Development Program AB20072003Innovation Project of Guilin University of Electronic Technology Graduate Education 2021YCXS172Young Teacher's Basic Ability Promotion Project of Guangxi 2021KY0224
6 · The paper itself

Abstract

backgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly infectious and concealed virus that causes pneumonia, severe acute respiratory syndrome, and even death. Although the epidemic has been controlled since the development of vaccines and quarantine measures, many people are still infected, particularly in third-world countries. Several methods have been developed for detection of SARS-CoV-2, but owing to its price and efficiency, the immune strip could be a better method for the third-world countries.

methodsIn this study, two antibodies were linked to latex microspheres, using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, as the bridge to decrease the cost further and improve the detection performance. The specificity of the lateral flow immunoassay strip (LFIA) was tested by several common viruses and respiratory bacterial infections. Besides, the reproducibility and stability of the LFIAs were tested on the same batch of test strips. Under optimal conditions, the sensitivity of LFIA was determined by testing different dilutions of the positive specimens.

resultsThe proposed LFIAs were highly specific, and the limit of detection was as low as 25 ng/mL for SARS-CoV-2 antigens. The clinical applicability was evaluated with 659 samples (230 positive and 429 negative samples) by using both LFIA and rRT-PCR. Youden's index (J) was used to assess the performance of these diagnostic tests. The sensitivity and specificity were 98.22% and 97.93%, respectively, and J is 0.9615. The sensitivity and specificity were 98.22% and 97.93%, respectively, and J is 0.9615. In addition, the consistency of our proposed LFIA was analyzed using Cohen's kappa coefficient (κ = 0.9620).

conclusionWe found disease stage, age, gender, and clinical manifestations have only a slight influence on the diagnosis. Therefore, the lateral flow immunoassay SARS-CoV-2 antigen test strip is suitable for point-of-care detection and provides a great application for SARS-CoV-2 epidemic control in the third-world countries.

Indexed as

Antigens, ViralCarbodiimidesCOVID-19 Serological TestingHumansImmunoassayLatexMethylaminesMicroscopy, Electron, ScanningMicrospheresPoint-of-Care SystemsReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSARS-CoV-2Sensitivity and SpecificitySuccinimides1-(3-dimethylaminopropyl)-dimethylaminopropyl-ethylcarbodiimide hydrochlorideAntigens, ViralCarbodiimidesLatexMethylaminesN-hydroxysuccinimideSuccinimidesEDC and NHSlatex microspheresSARS-CoV-2 antigen

Identifiers

PMID34741352
PMCPMC8646881
OpenAlexW3209021320

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.