Evidence map›Paper›PMID 34741115›Full record

ArticleCommunications biology2021

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Dennis Ma, Grace A Hernandez, Austin E Y T Lefebvre, Hamad Alshetaiwi, Kerrigan Blake, Kushal R Dave, Maha Rauf, Justice W Williams, Ryan T Davis, Katrina T Evans and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Advancements in Zebrafish Models for Breast Cancer Research: Unveiling Biomarkers, Targeted Therapies, and Personalized Medicine.Medical science monitor : international medical journal of experimental and clinical research · 2023
    Pooled it
  2. Article
  3. Mechanisms of breast cancer dormancy in bone metastasis.Clinical & experimental metastasis · 2026
    Review
  4. Review
  5. Article
  6. Article
  7. Mouse Models of Metastasis and Dormancy.Cold Spring Harbor perspectives in medicine · 2024
    Review
  8. Article
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Dennis Ma *Department of Biological Chemistry, University of California, Irvine, CA, USA.
Grace A Hernandez *Department of Physiology and Biophysics, University of California, Irvine, CA, USA.
Austin E Y T LefebvreDepartment of Biomedical Engineering, University of California, Irvine, CA, USA.
Hamad AlshetaiwiDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
Kerrigan BlakeCenter for Complex Biological Systems, University of California, Irvine, CA, USA.
Kushal R DaveDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.
Maha RaufDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
Justice W WilliamsDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
Ryan T DavisDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0003-0036-4085
Katrina T EvansDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.
Aaron LongworthDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0003-1893-3880
Madona Y G MasoudDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.
Regis LeeDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.
Robert A EdwardsDepartment of Pathology & Laboratory Medicine, University of California, Irvine, CA, USA.
Michelle A DigmanDepartment of Biomedical Engineering, University of California, Irvine, CA, USA.ORCID 0000-0003-4611-7100
Kai KessenbrockDepartment of Biological Chemistry, University of California, Irvine, CA, USA.ORCID 0000-0003-0410-6104
Devon A LawsonDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA. dalawson@uci.edu.ORCID 0000-0003-1692-0517

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
CARCINOGENESIST32CA009054 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI EDINGER, AIMEE L, FRUMAN, DAVID ALEXANDER · 1985 to 2025
$8.6M
Microenvironmental regulation of breast tumorigenesisR01CA234496 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Kai Kessenbrock · 2019 to 2026
$4.3M
Proteolytic regulation of Wnt signaling in breast cancer stem cellsR00CA181490 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI KESSENBROCK, KAI · 2016 to 2018
$730k
Single-cell analysis of intratumor heterogeneity in metastasisK22CA190511 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI LAWSON, DEVON A. · 2016 to 2018
$486k
American Cancer Society (American Cancer Society, Inc.) 132551-RSG-18-194-01-DDCAmerican Cancer Society (American Cancer Society, Inc.) 134389-RSG-20-039-01-DDCNCI NIH HHS K22 CA190511NCI NIH HHS P30 CA062203NCI NIH HHS R00 CA181490NCI NIH HHS R01 CA234496NCI NIH HHS T32 CA009054NIGMS NIH HHS P41 GM103540
6 · The paper itself

Abstract

Metastasis is a fatal disease where research progress has been hindered by a lack of authentic experimental models. Here, we develop a 3D tumor sphere culture-transplant system that facilitates the growth and engineering of patient-derived xenograft (PDX) tumor cells for functional metastasis assays in vivo. Orthotopic transplantation and RNA sequencing (RNA-seq) analyses show that PDX tumor spheres maintain tumorigenic potential, and the molecular marker and global transcriptome signatures of native tumor cells. Tumor spheres display robust capacity for lentiviral engineering and dissemination in spontaneous and experimental metastasis assays in vivo. Inhibition of pathways previously reported to attenuate metastasis also inhibit metastasis after sphere culture, validating our approach for authentic investigations of metastasis. Finally, we demonstrate a new role for the metabolic enzyme NME1 in promoting breast cancer metastasis, providing proof-of-principle that our culture-transplant system can be used for authentic propagation and engineering of patient tumor cells for functional studies of metastasis.

Indexed as

HeterograftsNeoplasm MetastasisXenograft Model Antitumor AssaysAnimalsBreast NeoplasmsDisease Models, AnimalFemaleMiceNeoplasms, ExperimentalTumor Microenvironment

Identifiers

PMID34741115
PMCPMC8571269

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.