ArticleScientific reports2021
Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Article
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- Overexpression of the Lipid Transfer Protein GenePlants (Basel, Switzerland) · 2025Article
- Analysis of full length transcriptome and resistance characteristics of Atraphaxis bracteata under drought.Scientific reports · 2025Article
- Insights into the molecular mechanisms of browning tolerance in luffa: a transcriptome and metabolome analysis.Frontiers in plant science · 2025Article
- Selection of Reference Genes for Expression Normalization by RT-qPCR inCurrent issues in molecular biology · 2024Article
- Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi.Scientific reports · 2023Article
- Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses.Scientific reports · 2022Article
- Analysis of controlling genes for tiller growth of Psathyrostachys juncea based on transcriptome sequencing technology.BMC plant biology · 2022Article
- Identification of Gene Biomarkers for Tigilanol Tiglate Content inMolecules (Basel, Switzerland) · 2022Article
- Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic mossFrontiers in plant science · 2022Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which S. pennata adapts to adverse desert environments at the molecular and genetic levels. In this study, eight candidate reference genes were identified from rhizosheath development transcriptome data from S. pennata, and their expression stability in the rhizosheaths at different development stages, in a variety of plant tissues, and under drought stress was evaluated using four procedures, including geNorm, NormFinder, BestKeeper, and RefFinder. The results showed that GAPDH and elF were the most stable reference genes under drought stress and in rhizosheath development, and ARP6 and ALDH were relatively stable in all plant tissues. In addition, elF was the most suitable reference gene for all treatments. Analysis of the consistency between the reverse transcription-quantitative PCR (RT-qPCR) and RNA sequencing data showed that the identified elF and GAPDH reference genes were stable during rhizosheath development. These results provide reliable reference genes for assuring the accuracy of RT-qPCR and offer a foundation for further investigations into the genetic responses of S. pennata to abiotic stress.
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