ArticleMolecular cell2021
Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Article in Molecular cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- Bacterial 3D genome architecture: organization, regulation, and synthetic biology applications.Genome biology · 2026Review
- SMC complex unidirectionally translocates DNA by coupling segment capture with an asymmetric kleisin path.eLife · 2026Article
- DNA deformability in sequence-dependent capture of E. coli gyrase.Nature communications · 2026Article
- Review
- Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.Communications biology · 2025Article
- Structural basis for Lamassu-based antiviral immunity and its evolution from DNA repair machinery.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bacterial chromosome conformation and cell-free gene expression in synthetic 2D compartments.Nature communications · 2025Article
- Chromosome segregation dynamics during the cell cycle of Staphylococcus aureus.Nature communications · 2025Article
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- Principles of bacterial genome organization, a conformational point of view.Molecular microbiology · 2025Review
- Nucleotide-induced hyper-oligomerization inactivates transcription termination factor ρ.Nature communications · 2025Article
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- In through the out door: A loop-binding-first model for topological cohesin loading.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Article
- MksB is a novel mycobacterial condensin that orchestrates spatiotemporal positioning of replication machinery.Scientific reports · 2024Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Loop extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex.bioRxiv : the preprint server for biology · 2024Article
- Activity of MukBEF for chromosome management ineLife · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- SMC-based immunity against extrachromosomal DNA elements.Biochemical Society transactions · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication terminus macrodomain is suppressed by the sequence-specific unloader MatP. Here, we present the complete atomic structure of MukBEF in complex with MatP and DNA as determined by electron cryomicroscopy (cryo-EM). The complex binds two distinct DNA double helices corresponding to the arms of a plectonemic loop. MatP-bound DNA threads through the MukBEF ring, while the second DNA is clamped by the kleisin MukF, MukE, and the MukB ATPase heads. Combinatorial cysteine cross-linking confirms this topology of DNA loop entrapment in vivo. Our findings illuminate how a class of near-ubiquitous DNA organizers with important roles in genome maintenance interacts with the bacterial chromosome.
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