Evidence map›Paper›PMID 34739601›Full record

ArticlePlanta2021

The regulation of DKGA2ox1 and miR171f_3 in scion dwarfing with Diospyros kaki Thunb. cv. 'Nan-tong-xiao-fang-shi' as interstocks.

Yuhan Dong, Xialin Ye, Lifang Cao, Xinyi Yu, Shenchun Qu

Abstract read
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In one paragraph

Article in Planta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yuhan DongCollege of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Xialin YeCollege of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Lifang CaoCollege of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Xinyi YuCollege of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. t2020066@njau.edu.cn.
Shenchun QuCollege of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. qscnj@njau.edu.cn.ORCID http://orcid.org/0000-0003-3615-037X
Nanjing Agricultural University · CN

Funding

Jiangsu Belt and Road innovation cooperation project BZ2019012Postgraduate Research&Practice Innovation Program of Jiangsu Province KYCX21_0606the Fundamental Research Funds for the Central Universities KYZZ201922The National Key R&D Program of China 2019YFD1000600
6 · The paper itself

Abstract

MAIN

conclusionHigh-throughput sequencing and yeast one and two-hybrid library screening reveal that DKGA2ox1 and miR171f_3 are involved in the regulation of scion dwarfing with 'Nan-tong-xiao-fang-shi' as interstocks. Diospyros kaki Thunb. cv. Nan-tong-xiao-fang-shi ('Nan-tong-xiao-fang-shi') interstocks play a critical role in the scion dwarfing. However, the understanding of the molecular signaling pathways that regulate the scion dwarfing with 'Nan-tong-xiao-fang-shi' as interstocks remain unclear. In this work, the regulatory network in the scion dwarfing with 'Nan-tong-xiao-fang-shi' as interstocks was identified. Using a yeast one-hybrid library screening, luciferase activity analysis, luciferase complementation imaging assays and GFP signal detection, a SPL transcription factor named Diospyros kaki SPL (DKSPL), potentially functioning as a transcriptional activator of the Diospyros kaki GA2ox1 (DKGA2ox1) gene, was identified as a key stimulating factor in the persimmon growth and development. The DKSPL was found in the nucleus, and might play a role in the transcriptional regulation system. A microRNA named miR171f_3 was identified, which might act as a negative regulator of Diospyros kaki SCR (DKSCR) in persimmon. The interactions between DKSCR and seven proteins were experimentally validated with a yeast two-hybrid library screening. Compared to the non-grafted wildtype persimmon, the tissue section of graft union healed well due to the increased expression of cinnamyl-alcohol dehydrogenase. These results indicate that DKGA2ox1 and miR171f_3 may co-promote the scion dwarfing by plant hormone signal transduction pathways.

Indexed as

DiospyrosMicroRNAsFruitPlant Growth RegulatorsTranscription FactorsMicroRNAsPlant Growth RegulatorsTranscription FactorsDwarfGibberellinGraftMicroRNAPersimmonYeast hybrid

Identifiers

PMID34739601
OpenAlexW3209826224

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.