Evidence map›Paper›PMID 34737378›Full record

ArticleCommunications biology2021

Picroscope: low-cost system for simultaneous longitudinal biological imaging.

Victoria T Ly, Pierre V Baudin, Pattawong Pansodtee, Erik A Jung, Kateryna Voitiuk, Yohei M Rosen, Helen Rankin Willsey, Gary L Mantalas, Spencer T Seiler, John A Selberg and 9 more

Open access · goldAbstract read
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 39 citations in OpenAlex.

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  17. IoT cloud laboratory: Internet of Things architecture for cellular biology.Internet of things (Amsterdam, Netherlands) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 3 institutions in 1 country.

Victoria T Ly *Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA. vily@ucsc.edu.ORCID http://orcid.org/0000-0003-4408-777X
Pierre V Baudin *Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Pattawong PansodteeDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Erik A JungDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Kateryna VoitiukDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.ORCID http://orcid.org/0000-0002-6392-5188
Yohei M RosenDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Helen Rankin WillseyDepartment of Psychiatry and Behavioral Sciences, Weill Institute for Neurosciences, University of California San Francisco, San Francisco, CA, 94143, USA.
Gary L MantalasDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Spencer T SeilerDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
John A SelbergDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Sergio A CorderoDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Jayden M RossThe Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, 94143, USA.
Marco RolandiDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.
Alex A PollenThe Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0003-3263-8634
Tomasz J NowakowskiThe Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, 94143, USA.
David HausslerDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.ORCID http://orcid.org/0000-0003-1533-4575
Mohammed A Mostajo-RadjiThe Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0002-1634-7514
Sofie R SalamaDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA.ORCID http://orcid.org/0000-0001-6999-7193
Mircea TeodorescuDepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, 95060, USA. mteodore@ucsc.edu.ORCID http://orcid.org/0000-0001-7085-5248
University of California, Santa Cruz · USUniversity of California, San Francisco · USHoward Hughes Medical Institute · US

Funding

The Psychiatric Cell Map Initiative: Connecting Genomics, Subcellular Networks, and Higher Order PhenotypesU01MH115747 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KAMPMANN, MARTIN, KROGAN, NEVAN J · 2018 to 2022
$19.6M
NRSA Training CoreTL1TR001871 · NCATS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KANAYA, ALKA M., SOMSOUK, MA · 2016 to 2025
$10.1M
Dissecting the role of NOTCH2NL genes in human brain development and neurological disorders associated with chromosome 1q21.1 distal duplications and deletions.R01MH120295 · NIMH · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI SALAMA, SOFIE REDA · 2020 to 2024
$3.7M
UC Santa Cruz Training Program In Genomic SciencesT32HG008345 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI BROOKS, ANGELA NORIE, GREEN, RICHARD EDWARD · 2015 to 2020
$1.4M
NCATS NIH HHS TL1 TR001871NHGRI NIH HHS T32 HG008345NIMH NIH HHS R01 MH120295NIMH NIH HHS U01 MH115747
6 · The paper itself

Abstract

Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Here, we propose the Picroscope, which is the first low-cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. Here, we use this system in a range of applications. We gathered longitudinal whole organism image data for frogs, zebrafish, and planaria worms. We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. Using this tool, we can measure the behavior of entire organisms or individual cells over long-time periods.

Indexed as

MammalsPlanariansXenopusZebrafishAnimalsBehavior, AnimalImaging, Three-DimensionalOrganoids

Identifiers

PMID34737378
PMCPMC8569150
OpenAlexW3209516162

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.