ArticleACS chemical biology2021
Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells.
Article in ACS chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Quantitative Characterization of Microtubule Ultrastructure Based on Single-Molecule Localization Microscopy.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- Illuminating microtubule functions with small molecules: past, present and future of fluorescent tubulin-binding probes.RSC chemical biology · 2026Review
- A high-affinity split-HaloTag for live-cell protein labeling.Nature communications · 2026Article
- Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells.Nature communications · 2025Article
- Gradual labeling with fluorogenic probes: A general method for MINFLUX imaging and tracking.Science advances · 2025Article
- Review
- MINFLUX Nanoscopy: A "Brilliant" Technique Promising Major Breakthrough.Microscopy research and technique · 2025Review
- Microtubules as a versatile reference standard for expansion microscopy.Communications biology · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy.ACS central science · 2023Article
- A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy.Nature communications · 2023Article
- Structural studies of protein-nucleic acid complexes: A brief overview of the selected techniques.Computational and structural biotechnology journal · 2023Review
- Role of motor proteins in human cancers.Saudi journal of biological sciences · 2022Review
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (International ed. in English) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- At the molecular resolution with MINFLUX?Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report a small molecule tubulin probe for single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy and MINFLUX nanoscopy, which can be used in living and fixed cells. We explored a series of taxane derivatives containing spontaneously blinking far-red dye hydroxymethyl silicon-rhodamine (HMSiR) and found that the linker length profoundly affects the probe permeability and off-targeting in living cells. The best performing probe, HMSiR-tubulin, is composed of cabazitaxel and the 6'-regioisomer of HMSiR bridged by a C6 linker. Microtubule diameter of ≤50 nm was routinely measured in SMLM experiments on living and fixed cells. HMSiR-tubulin allows a complementary use of different nanoscopy techniques for investigating microtubule functions and developing imaging methods. For the first time, we resolved the inner microtubule diameter of 16 ± 5 nm by optical nanoscopy and thereby demonstrated the utility of a self-blinking dye for MINFLUX imaging.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.