Evidence map›Paper›PMID 34734515›Full record

ArticleJournal of biomedical optics2021

Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis.

Joshua Philippe Olorocisimo, Jeric Briones, Kiyotaka Sasagawa, Makito Haruta, Hironari Takehara, Hiroyuki Tashiro, Norihiro Ishida-Kitagawa, Yasumasa Bessho, Jun Ohta

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Joshua Philippe OlorocisimoNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Jeric BrionesNara Institute of Science and Technology, Mathematical Informatics Laboratory, Division of Informati, Japan.
Kiyotaka SasagawaNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Makito HarutaNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Hironari TakeharaNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Hiroyuki TashiroNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Norihiro Ishida-KitagawaNara Institute of Science and Technology, Gene Regulation Research Laboratory, Division of Biologica, Japan.
Yasumasa BesshoNara Institute of Science and Technology, Gene Regulation Research Laboratory, Division of Biologica, Japan.
Jun OhtaNara Institute of Science and Technology, Photonics Device Science Laboratory, Division of Materials, Japan.
Nara Institute of Science and Technology · JPAdvanced Telecommunications Research Institute International · JPKyushu University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

significanceGene expression analysis is an important fundamental area of biomedical research. However, live gene expression imaging has proven challenging due to constraints in conventional optical devices and fluorescent reporters.

aimOur aim is to develop smaller, more cost-effective, and versatile imaging capabilities compared with conventional devices. Bioluminescence reporter-based gene expression analysis was targeted due to its advantages over fluorescence-based imaging. APPROACH: We created a small compact imaging system using micro-CMOS image sensors (μCIS). The μCIS model had an improved pixel design and a patterned absorption filter array to detect the low light intensity of bioluminescence.

resultsThe device demonstrated lower dark current, lower temporal noise, and higher sensitivity compared with previous designs. The filter array enabled us to subtract dark current drift and attain a clearer light signal. These improvements allowed us to measure bioluminescence reporter-based gene expression in living mammalian cells.

conclusionUsing our μCIS system for bioluminescence imaging in the future, the device can be implanted in vivo for simultaneous gene expression imaging, behavioral analysis, and optogenetic modulation.

Indexed as

Luminescent MeasurementsAnimalsGene ExpressionGenes, Reporterbioluminescencebiophotonicsgene expressionimage sensorsluciferasemicro-CMOS

Identifiers

PMID34734515
PMCPMC8564164
OpenAlexW3208597510

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.