ArticleScientific reports2021
The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epigenetic signatures relating to disease-associated genotypic burden in familial risk of bipolar disorder.Translational psychiatry · 2022Pooled it
- A DNA Methylation-based algorithm Improves Lung Cancer risk prediction in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial.Lung cancer (Amsterdam, Netherlands) · 2026Article
- Impact of tobacco smoking on estimated telomere shortening and epigenetic age acceleration among human blood immune cell types.Clinical epigenetics · 2026Article
- EpiSmokEr2: a robust epigenetic classifier for smoking status inference using Illumina EPIC methylation data.Epigenomics · 2026Article
- Article
- Epigenetic biomarkers predict macrovascular events in individuals with type 2 diabetes.Cell reports. Medicine · 2025Article
- The Effect of Clinical Factors on the Reversion of Cg05575921 Methylation in Smoking Cessation.Epigenomes · 2025Article
- Blood-Based Epigenetic Biomarkers Associated With Incident Chronic Kidney Disease in Individuals With Type 2 Diabetes.Diabetes · 2025Article
- Genetic and epigenetic biomarkers in human biomonitoring: why needed and how can Oxford Nanopore sequencing contribute?Frontiers in public health · 2025Review
- Te Oranga Pūkahukahu research programme: intentional steps towards a national equity-focused lung cancer screening programme in Aotearoa New Zealand.Journal of the Royal Society of New Zealand · 2025Article
- Biochemical Diagnosis in Substance and Non-substance Addiction.Advances in experimental medicine and biology · 2025Review
- Two Clinically Implementable Digital PCR Assessments of DNA Methylation for Diagnosing Heavy Alcohol Consumption.Epigenomes · 2024Review
- Article
- Article
- The Reversion of the Epigenetic Signature of Coronary Heart Disease in Response to Smoking Cessation.Genes · 2023Article
- Article
- Epigenetic biomarkers for smoking cessation.Addiction neuroscience · 2023Article
- Promising applications of human-derived saliva biomarker testing in clinical diagnostics.International journal of oral science · 2023Review
- Saliva-based microfluidic point-of-care diagnostic.Theranostics · 2023Review
- Using Cg05575921 methylation to predict lung cancer risk: a potentially bias-free precision epigenetics approach.Epigenetics · 2022Article
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8 authors.
Funding
Abstract
Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to assess and monitor smoking status. To better define this relationship, we conducted a joint analysis of methylation sensitive PCR digital (MSdPCR) assessments of cg05575921 methylation in whole blood and/or saliva DNA to smoking using samples from 421 smokers and 423 biochemically confirmed non-smokers from 4 previously published studies. We found that cg05575921 methylation manifested a curvilinear dose dependent decrease in response to increasing cigarette consumption. In whole blood DNA, the Receiver Operating Characteristic (ROC) Area Under the Curve (AUC) of cg05575921 methylation for predicting daily smoking status was 0.98. In saliva DNA, the gross AUC was 0.91 with correction for cellular heterogeneity improving the AUC to 0.94. Methylation status was significantly associated with the Fagerstrom Test for Nicotine Dependence score, but with significant sampling heterogeneity. We conclude that MSdPCR assessments of cg05575921 methylation are a potentially powerful, clinically implementable tool for the assessment and management of smoking.
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