Evidence map›Paper›PMID 34732754›Full record

ArticleScientific reports2021

The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death.

Junior Bernardo Molina-Hernandez, Antonio Aceto, Tonino Bucciarelli, Domenico Paludi, Luca Valbonetti, Katiuscia Zilli, Luca Scotti, Clemencia Chaves-López

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
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  3. Eco-Friendly Access to Indenodiazepinones fromACS medicinal chemistry letters · 2026
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  6. Article
  7. Biocidal Properties of New Silver Nanoparticles Argirium SUNcNanomaterials (Basel, Switzerland) · 2025
    Article
  8. Article
  9. Transcriptome analysis ofFrontiers in microbiology · 2025
    Article
  10. Article
  11. Review
  12. Antifungal Mechanism ofMolecules (Basel, Switzerland) · 2024
    Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Silver nanoclusters with AgApplied microbiology and biotechnology · 2023
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Junior Bernardo Molina-Hernandez *Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Antonio AcetoDepartment of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Tonino BucciarelliDepartment of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Domenico PaludiFaculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
Luca ValbonettiFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Katiuscia ZilliIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale, Teramo, Italy.
Luca Scotti *Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. l.scotti@unich.it.
Clemencia Chaves-López *Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
University of Teramo · ITUniversity of Chieti-Pescara · ITIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale · IT

Funding

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale ex60%Università degli Studi di Teramo ex60%Università degli Studi G. d'Annunzio Chieti - Pescara ex60%
6 · The paper itself

Abstract

This work highlights how our silver ultra nanoclusters (ARGIRIUM-SUNc) hand-made synthesized, are very useful as a bactericide and anti-biofilm agent. The Argirium-SUNc effective antibacterial concentrations are very low (< 1 ppm) as compared to the corresponding values reported in the literature. Different bacterial defense mechanisms are observed dependent on ARGIRIUM-SUNc concentrations. Biochemical investigations (volatilome) have been performed to understand the pathways involved in cell death. By using fluorescence techniques and cell viability measurements we show, for the first time, that membrane depolarization and calcium intracellular level are both primary events in bacteria death. The ARGIRIUM-SUNc determined eradication of different biofilm at a concentration as low as 0.6 ppm. This suggests that the effect of the nanoparticles follows a common mechanism in different bacteria. It is highly probable that the chemical constitution of the crosslinks could be a key target in the disrupting mechanism of our nanoparticles. Since the biofilms and their constituents are essential for bacterial survival in contact with humans, the silver nanoparticles represent a logical target for new antibacterial treatments.

Indexed as

Microbial Sensitivity TestsAcinetobacter baumanniiAnti-Bacterial AgentsBiofilmsCalciumCell SurvivalEnterobacterEnterococcus faeciumGlutathioneKineticsKlebsiella pneumoniaeMembrane PotentialsMetal NanoparticlesMicroscopy, Electron, TransmissionMicroscopy, FluorescenceNanotechnologyAnti-Bacterial AgentsCalciumGlutathioneReactive Oxygen SpeciesSilver

Identifiers

PMID34732754
PMCPMC8566483
OpenAlexW3208684361

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.