ArticleMicrobiome2021
Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption.
Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Effect of Fructooligosaccharides Supplementation on the Gut Microbiota in Human: A Systematic Review and Meta-Analysis.Nutrients · 2022Pooled it
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- Augmentation of psychrophilic anaerobic digestion with psychrotolerantFrontiers in microbiology · 2026Article
- High-altitude mountaineering induces adaptive gut microbiome shifts associated with dietary intake and performance markers.Scientific reports · 2025Article
- The Hidden Microbial World in the Gut of the Terrestrial SnailMicroorganisms · 2025Article
- Breaking down barriers: is intestinal mucus degradation byInfection and immunity · 2025Review
- A longitudinal investigation of gut microbiota dynamics in laying hens from birth to egg-laying stages.Animal bioscience · 2025Article
- Microbiota-gut-brain Axis: Novel Potential Pathways for Developing Antiepileptogenic Drugs.Current neuropharmacology · 2025Review
- Collecting the evidence: mechanistic insights intoFrontiers in immunology · 2025Review
- Coupled Effect of Nutritional Food Molecules andFoods (Basel, Switzerland) · 2024Article
- Interactions between Dietary Antioxidants, Dietary Fiber and the Gut Microbiome: Their Putative Role in Inflammation and Cancer.International journal of molecular sciences · 2024Review
- Calcium-rich dairy matrix protects better than mineral calcium against colonic luminal haem-induced alterations in male rats.NPJ science of food · 2024Article
- Utilization of diverse oligosaccharides for growth by Bifidobacterium and Lactobacillus species and their in vitro co-cultivation characteristics.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Nutritional Support: The Use of Antioxidants in Inflammatory Bowel Disease.International journal of molecular sciences · 2024Review
- Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders.Scientific reports · 2024Article
- SynergisticMicrobiome research reports · 2024Review
- Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population.Cancers · 2023Article
- Blocked conversion of Lactobacillus johnsonii derived acetate to butyrate mediates copper-induced epithelial barrier damage in a pig model.Microbiome · 2023Article
- Effects of Menaquinone-7 on the Bone Health of Growing Rats under Calcium Restriction: New Insights from Microbiome-Metabolomics.Nutrients · 2023Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobacilli, and are reported to elicit several health benefits. The combination of dietary FOS and inulin with calcium phosphate was reported to stimulate commensal Lactobacillus populations and protect the host against pathogenic Enterobacteriaceae, but little is known about the effects of GOS in diets with a different level of calcium phosphate.
methodsWe investigated the microbiome changes elicited by dietary supplementation with GOS or inulin using diets with high (100 mmol/kg) and low (30 mmol/kg) calcium phosphate levels in adult Wistar rats. Rats were acclimatized to the respective experimental diets for 14 days, after which fecal material was collected, DNA was extracted from fecal material, and the V3‑V4 region of the bacterial 16S rRNA gene was amplified with PCR, followed by microbial composition analysis. In tandem, the organic acid profiles of the fecal material were analyzed.
resultsFeeding rats non-supplemented (no prebiotic-added) diets revealed that diets rich in calcium phosphate favored members of the Firmicutes and increased fecal lactic, succinic, acetic, propionic, and butyric acid levels. In contrast, relatively low dietary calcium phosphate levels promoted the abundance of mucin degrading genera like Akkermansia and Bacteroides, and resulted in increased fecal propionic acid levels and modest increases in lactic and butyric acid levels. Irrespective of the calcium phosphate levels, supplementation with GOS or inulin strongly stimulated Bifidobacterium, while only high calcium phosphate diets increased the endogenous Faecalibaculum populations.
conclusionsDespite the prebiotic's substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level. Therefore, our study implies that the collection of detailed diet information including micronutrient balance is necessary to correctly assess diet-driven microbiota analysis. Video Abstract.
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