Evidence map›Paper›PMID 34731637›Full record

ArticleCell reports2021

Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-A

Catalina Salinas-Luypaert, Praveen Kumar Allu, Glennis A Logsdon, Jennine M Dawicki-McKenna, Craig W Gambogi, Daniele Fachinetti, Ben E Black

Open access · goldAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Catalina Salinas-LuypaertInstitut Curie, PSL University, CNRS, UMR 144, 26 rue d'Ulm, 75005, Paris, France.
Praveen Kumar AlluDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Glennis A LogsdonDepartment of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA.
Jennine M Dawicki-McKennaDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Craig W GambogiDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Graduate Program in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
Daniele FachinettiInstitut Curie, PSL University, CNRS, UMR 144, 26 rue d'Ulm, 75005, Paris, France. Electronic address: daniele.fachinetti@curie.fr.
Ben E BlackDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Graduate Program in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: blackbe@pennmedicine.upenn.edu.
University of Pennsylvania · USCentre National de la Recherche Scientifique · FRUniversity City Science Center · USUniversity of Washington · US

Funding

Centromere identity, strength, and regulationR35GM130302 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Ben E. Black · 2019 to 2026
$4.3M
NIGMS NIH HHS R35 GM130302
6 · The paper itself

Abstract

Functional tags are ubiquitous in cell biology, and for studies of one chromosomal locus, the centromere, tags have been remarkably useful. The centromere directs chromosome inheritance at cell division. The location of the centromere is defined by a histone H3 variant, CENP-A. The regulation of the chromatin assembly pathway essential for centromere inheritance and function includes posttranslational modification (PTM) of key components, including CENP-A itself. Others have recently called into question the use of functional tags, with the claim that at least two widely used tags obscured the essentiality of one particular PTM, CENP-A

Indexed as

Genetic TechniquesCell Line, TumorCell SurvivalCentromereCentromere Protein AChromatinChromatin Assembly and DisassemblyColonic NeoplasmsGene EditingHumansLysineMutationRetinal Pigment EpitheliumUbiquitinationCENPA protein, humanCentromere Protein AChromatinLysineCENP-Acentromerefunctional protein tagsgenome editinghistone variantmitosisposttranslational modificationubiquitin

Identifiers

PMID34731637
PMCPMC8643106
OpenAlexW3210510958

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.