Evidence map›Paper›PMID 34730351›Full record

ArticleBioconjugate chemistry2021

Protein Modification via Mild Photochemical Isomerization of Triazenes to Release Aryl Diazonium Ions.

Garrett J Davis, Julia A Townsend, Madeline G Morrow, Mohamed Hamie, Abigail J Shepard, Chih-Chieh Hsieh, Michael T Marty, John C Jewett

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Garrett J DavisDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Julia A TownsendDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Madeline G MorrowDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Mohamed HamieDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Abigail J ShepardDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Chih-Chieh HsiehDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Michael T MartyDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.ORCID 0000-0001-8115-1772
John C JewettDepartment of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 East University Boulevard, Tucson, Arizona 85721, United States.ORCID 0000-0001-5822-4080

Funding

CHEMISTRY-BIOLOGY INTERFACE TRAINING PROGRAMT32GM008804 · NIGMS · UNIVERSITY OF ARIZONA · PI MONTFORT, WILLIAM R. · 2003 to 2023
$3.9M
Unravelling Membrane Protein-Lipid Interactions using Nanodiscs and Mass SpectrometryR35GM128624 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Michael T Marty · 2018 to 2026
$3.6M
NIGMS NIH HHS R35 GM128624NIGMS NIH HHS T32 GM008804
6 · The paper itself

Abstract

This work describes the development of phenyl diazenyl piperidine triazene derivatives that can be activated to release aryl diazonium ions for labeling of proteins using light. These probes show marked bench stability at room temperature and can be photoisomerized via low-intensity UVA irradiation at physiological pH. Upon isomerization, the triazenes are rendered more basic and readily protonate to release reactive aryl diazonium ions. It was discovered that the intensity and duration of the UV light was essential to the observed diazonium ion reactivity in competition with the traditionally observed photolytic radical pathways. The combination of their synthetic efficiency coupled with their overall stability makes triazenes an attractive candidate for use in bioconjugation applications. Bioorthogonal handles on the triazenes are used to demonstrate the ease by which proteins can be modified.

Indexed as

Diazonium CompoundsTriazenesIonsIsomerismPhotochemical ProcessesProteinsUltraviolet RaysDiazonium CompoundsIonsProteinsTriazenes

Identifiers

PMID34730351
PMCPMC8820681

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.