Evidence map›Paper›PMID 34727202›Full record

Trial reportEuropean journal of nutrition2022

Higher bacterial DNAemia can affect the impact of a polyphenol-rich dietary pattern on biomarkers of intestinal permeability and cardiovascular risk in older subjects.

Giorgio Gargari, Valentina Taverniti, Cristian Del Bo', Stefano Bernardi, Nicole Hidalgo-Liberona, Tomás Meroño, Cristina Andres-Lacueva, Paul A Kroon, Antonio Cherubini, Patrizia Riso and 1 more

Open access · greenAbstract readRandomized Controlled Trial
PubMed Publisher
In one paragraph

Trial report in European journal of nutrition, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 2 pooled it
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 2 syntheses or guidelines pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Review
  5. Article
  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Giorgio GargariDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy.
Valentina TavernitiDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy.
Cristian Del Bo'Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy.
Stefano BernardiBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Innovation Network (XIA), University of Barcelona, Barcelona, Spain.
Nicole Hidalgo-LiberonaBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Innovation Network (XIA), University of Barcelona, Barcelona, Spain.
Tomás MeroñoBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Innovation Network (XIA), University of Barcelona, Barcelona, Spain.
Cristina Andres-LacuevaBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Innovation Network (XIA), University of Barcelona, Barcelona, Spain.
Paul A KroonQuadram Institute Bioscience, Norwich Research Park, Norwich, UK.
Antonio CherubiniGeriatria, Accettazione geriatrica e Centro di Ricerca Per l'invecchiamento. IRCCS INRCA, Ancona, Italy.
Patrizia RisoDivision of Human Nutrition, Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy.
Simone GuglielmettiDivision of Food Microbiology and Bioprocesses, Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy. simone.guglielmetti@unimi.it.ORCID http://orcid.org/0000-0002-8673-8190
University of Milan · ITInstituto de Salud Carlos III · ESIstituti di Ricovero e Cura a Carattere Scientifico · ITNorwich Research Park · GB

Funding

Joint Programming Initiative A healthy diet for a healthy life BBSRC (U.K., BB/R012512/1)Joint Programming Initiative A healthy diet for a healthy life MINECO (Spain, PCIN-2015-238)Joint Programming Initiative A healthy diet for a healthy life Mipaaft (Italy, D.M. 8245/7303/ 2016)
6 · The paper itself

Abstract

purposeAging can be characterized by increased systemic low-grade inflammation, altered gut microbiota composition, and increased intestinal permeability (IP). The intake of polyphenol-rich foods is proposed as a promising strategy to positively affect the gut microbiota-immune system-intestinal barrier (IB) axis. In this context, we tested the hypothesis that a PR-dietary intervention would affect the presence of bacterial factors in the bloodstream of older adults.

methodsWe collected blood samples within a randomized, controlled, crossover intervention trial in which older volunteers (n = 51) received a polyphenol-enriched and a control diet. We quantified the presence of bacterial DNA in blood by qPCR targeting the 16S rRNA gene (16S; bacterial DNAemia). Blood DNA was taxonomically profiled via 16S sequencing.

resultsHigher blood 16S levels were associated with higher BMI and markers of IP, inflammation, and dyslipidemia. PR-intervention did not significantly change bacterial DNAemia in the older population (P = 0.103). Nonetheless, the beneficial changes caused by the polyphenol-enriched diet were greatest in participants with higher bacterial DNAemia, specifically in markers related to IP, inflammation and dyslipidemia, and in fecal bacterial taxa. Finally, we found that the bacterial DNA detected in blood mostly belonged to γ-Proteobacteria, whose abundance significantly decreased after the polyphenol-rich diet in subjects with higher bacterial DNAemia at baseline.

conclusionsThis study shows that older subjects with higher bacterial DNAemia experienced a beneficial effect from a polyphenol-rich diet. Bacterial DNAemia may be a further relevant marker for the identification of target populations that could benefit more from a protective dietary treatment. REGISTRATION: This trial was retrospectively registered at www.isrctn.org (ISRCTN10214981) on April 28, 2017.

Indexed as

Cardiovascular DiseasesPolyphenolsAgedBiomarkersDietFecesHeart Disease Risk FactorsHumansPermeabilityRisk FactorsRNA, Ribosomal, 16SBiomarkersPolyphenolsRNA, Ribosomal, 16SInterleukin-6MaPLE projectPseudomonasZonulinγ-Proteobacteria

Identifiers

PMID34727202
OpenAlexW3209333658

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.