Evidence map›Paper›PMID 34726737›Full record

ArticlePlant physiology2022

Organizing your space: The potential for integrating spatial transcriptomics and 3D imaging data in plants.

Kevin L Cox, Sai Guna Ranjan Gurazada, Keith E Duncan, Kirk J Czymmek, Christopher N Topp, Blake C Meyers

Open access · bronzeAbstract read
In one paragraph

Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Kevin L CoxDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0003-2524-8988
Sai Guna Ranjan GurazadaDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0002-3084-5683
Keith E DuncanDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0002-0833-190X
Kirk J CzymmekDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0002-5471-7395
Christopher N ToppDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0001-9228-6752
Blake C MeyersDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.ORCID 0000-0003-3436-6097
Donald Danforth Plant Science Center · USBiotechnology Institute · USHoward Hughes Medical Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant cells communicate information for the regulation of development and responses to external stresses. A key form of this communication is transcriptional regulation, accomplished via complex gene networks operating both locally and systemically. To fully understand how genes are regulated across plant tissues and organs, high resolution, multi-dimensional spatial transcriptional data must be acquired and placed within a cellular and organismal context. Spatial transcriptomics (ST) typically provides a two-dimensional spatial analysis of gene expression of tissue sections that can be stacked to render three-dimensional data. For example, X-ray and light-sheet microscopy provide sub-micron scale volumetric imaging of cellular morphology of tissues, organs, or potentially entire organisms. Linking these technologies could substantially advance transcriptomics in plant biology and other fields. Here, we review advances in ST and 3D microscopy approaches and describe how these technologies could be combined to provide high resolution, spatially organized plant tissue transcript mapping.

Indexed as

Spatial AnalysisTranscriptomeGene Expression Regulation, PlantGenes, PlantImaging, Three-DimensionalMicroscopy, FluorescencePlant Physiological PhenomenaPlantsSignal TransductionSingle-Cell Analysis

Identifiers

PMID34726737
PMCPMC8825300
OpenAlexW3208592229

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.