ArticleChemSusChem2022
Sustainable Flow-Synthesis of (Bulky) Nucleoside Drugs by a Novel and Highly Stable Nucleoside Phosphorylase Immobilized on Reusable Supports.
Article in ChemSusChem, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Going full circle: dynamic covalent enzyme immobilisationChemical science · 2026Article
- Integrating biocatalysis with continuous flow: current status, challenges, and future perspectives.Journal of advanced research · 2026Review
- Advances and Challenges in Bioprocess Optimization for the Synthesis of Sugar Nucleotides.Chembiochem : a European journal of chemical biology · 2025Review
- Light-Driven Photobiocatalytic Oxyfunctionalization in a Continuous Reactor System without External Oxygen Supply.ACS sustainable chemistry & engineering · 2025Article
- Pharmaceutical applications of halophilic enzymes.Heliyon · 2025Review
- A Comprehensive Guide to Enzyme Immobilization: All You Need to Know.Molecules (Basel, Switzerland) · 2025Review
- Immobilized Nucleoside 2'-Deoxyribosyltransferases from Extremophiles for Nucleoside Biocatalysis.ACS omega · 2025Article
- Synthesis of Substituted 1,2,4-Triazole-3-Thione Nucleosides UsingBiomolecules · 2024Article
- Supramolecular Ionic Liquid Gels for Enzyme Entrapment.ACS sustainable chemistry & engineering · 2023Article
- Halomonas elongata: a microbial source of highly stable enzymes for applied biotechnology.Applied microbiology and biotechnology · 2023Review
- A selective and atom-economic rearrangement of uridine by cascade biocatalysis for production of pseudouridine.Nature communications · 2023Article
- Production of Modified Nucleosides in a Continuous Enzyme Membrane Reactor.International journal of molecular sciences · 2023Article
- Biocatalytic Synthesis of Antiviral Nucleosides, Cyclic Dinucleotides, and Oligonucleotide Therapies.JACS Au · 2023Review
- A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs in continuous flow.Catalysis science & technology · 2022Article
- Sustainable Flow-Synthesis of (Bulky) Nucleoside Drugs by a Novel and Highly Stable Nucleoside Phosphorylase Immobilized on Reusable Supports.ChemSusChem · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The continuous synthesis of valuable nucleoside drugs was achieved in up to 99 % conversion by using a novel halotolerant purine nucleoside phosphorylase from Halomonas elongata (HePNP). HePNP showed an unprecedented tolerance to DMSO, usually required for substrate solubility, and could be immobilized on agarose microbeads through disulfide bonds, via a genetically fused Cystag. This covalent yet reversible binding chemistry showcased the reusability of agarose microbeads in a second round of enzyme immobilization with high reproducibility, reducing waste and increasing the sustainability of the process. Finally, the flow synthesis of a Nelarabine analogue (6-O-methyl guanosine) was optimized to full conversion on a 10 mm scale within 2 min residence time, obtaining the highest space-time yield (89 g L
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.