Evidence map›Paper›PMID 34725717›Full record

ArticleCell and tissue research2022

Expression pattern of alkB homolog 5 in goat testis and its role in spermatogonial stem cells.

Zifei Liu, Yu Cai, Mingtian Deng, Dongxu Li, Qingqing Leng, Liangyue Shi, Yutong Tang, Feng Wang, Yongjie Wan

Abstract read
PubMed Publisher
In one paragraph

Article in Cell and tissue research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Comprehensive Analysis of the Transcriptome-Wide mInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Zifei LiuJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Yu CaiJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Mingtian DengJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Dongxu LiJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Qingqing LengJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Liangyue ShiJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Yutong TangJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Feng WangJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China.
Yongjie WanJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang, Nanjing, Jiangsu, 210095, China. wanyongjie@njau.edu.cn.ORCID http://orcid.org/0000-0001-6846-3755
Nanjing Agricultural University · CN

Funding

China Postdoctoral Science Foundation 2019M661869Fundamental Research Funds for the Central Universities KYZ201855National Natural Science Foundation of China 31972569National Natural Science Foundation of China 32172735Natural Science Foundation of Jiangsu Province, China BK20200563
6 · The paper itself

Abstract

RNA N6-methyladenosine (m6A) is essential for many bioprocesses in many species, but its role in goat testis development remains elusive, especially alkB homolog 5 (ALKBH5), one of the m6A demethylases. To this end, nine healthy Haimen goats of different ages were chosen randomly to provide testes. The results showed that the expression level of ALKBH5 was increased significantly (P < 0.05) in the 9-month group compared with the 0-day and 3-month groups, and ALKBH5 was located in goat spermatocytes with the highest expression level compared with Leydig cells and Sertoli cells. Thus, pcDNA3.1-ALKBH5 was constructed to explore the influences of the ALKBH5 increase in goat spermatogonial stem cells (SSC) in vitro. The results showed that the expression level of ALKBH5 in SSC transfected with pcDNA3.1-ALKBH5 (OE_ALKBH5) was significantly increased (P < 0.001) compared with that in SSC transfected with pcDNA3.1-EGFP (EGFP). With ALKBH5 overexpression in SSC, flow cytometry analysis showed that cells at G1 phase were significantly reduced (P < 0.01), while cells at S phase significantly increased (P < 0.01), and cell apoptosis was inhibited. Accordingly, the mRNA degradation of CCND1, CCNE1, and BCL2 was suppressed with ALKBH5 overexpression in SSC after treatment with actinomycin D. Furthermore, the mRNA levels of pluripotency maintenance- and cell differentiation-associated genes were changed between the two groups. Overall, the results indicated the crucial role of ALKBH5 during Haimen goat testis development. The results of this study provide a theoretical basis and technical means for RNA methylation participating in goat testis development.

Indexed as

Adult Germline Stem CellsAlkB EnzymesAnimalsCell DifferentiationGoatsHumansMaleSpermatogoniaTestisTransfectionAlkB EnzymesALKBH5Haimen goatRNA methylationSpermatogonial stem cellsTestis

Identifiers

PMID34725717
OpenAlexW3210633027

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.