Evidence map›Paper›PMID 34724861›Full record

ArticleCell cycle (Georgetown, Tex.)2021

Exosomal lncAY927529 enhances prostate cancer cell proliferation and invasion through regulating bone microenvironment.

Qi Li, Jinhao Hu, Yibo Shi, Mulun Xiao, Tianxiang Bi, Chaoliang Wang, Liang Yan, Xiaoyan Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

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  14. Targeting PI3K/Akt signaling in prostate cancer therapy.Journal of cell communication and signaling · 2023
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  16. The diagnostic role and mechanistic functions of exosomal lncRNAs in prostate cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Qi LiDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.ORCID 0000-0001-5666-2079
Jinhao HuDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Yibo ShiDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Mulun XiaoDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Tianxiang BiDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Chaoliang WangDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Liang YanDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Xiaoyan LiDepartment of Neonatel Intensive Care Unit, Zhengzhou First People's Hospital, Zhengzhou, China.
First Affiliated Hospital of Zhengzhou University · CNZhengzhou People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes mediate the interaction between cancer cells and their microenvironment, and play a key role in tumor development. Although exosomes can package lncRNAs to mediate extracellular communication, the role of exosomal lncRNA AY927529 in prostate cancer (PCa) remains unclear. Exosomes were extracted from normal human prostatic epithelial cell lines (BPH-1 and RWPE-1) and PCa cell lines (VCaP and LNCaP, DU145, PC3) by ultrahigh speed centrifugation. Results of Western blot indicated that Alix, HSC70 and TSGl01 protein levels were upregulated in exosomes derived from PCa cells. LncAY927529 level was upregulated in PCa cells and exosomes derived from PCa patient serum and human PCa cells. CCK-8, Transwell and Flow cytometry assays demonstrated that bone marrow stromal cell line (ST2) conditioned medium (ST2-CM), treated with exosomes derived from PCa cells with high lncAY927529 level, promoted proliferation and invasion of PC3 and DU145 cells, and inhibited cell apoptosis. RT-qPCR assay indicated that lncAY927529 level was downregulated in PC3 and DU145 cells, exosomes derived from PCa cells (PCa-Exo) and ST2-CM treated with PCa-Exo with low expression of lncAY927529, and overexpression of lncAY927529 had the opposite results. In addition, Western blot assay showed that the autophagy related protein LC3II level was increased in ST2 cells treated with exosomes derived from DU145 cells with high expression of lncAY927529, and LC3I protein level was decreased. CXCL14 acted as a RNA-binding protein of lncAY927529, and exosome-mediated lncAY927529 positively regulated CXCL14 levels in ST2 cells. In general, exosome-mediated lncAY927529 could promote PCa cell proliferation and invasion by regulating bone microenvironment, suggesting that exosomal lncAY927529 may be a potential molecular diagnostic marker of PCa.

Indexed as

ExosomesMicroRNAsProstatic HyperplasiaProstatic NeoplasmsRNA, Long NoncodingBone and BonesCell Line, TumorCell MovementCell ProliferationHumansMaleProstateTumor MicroenvironmentMicroRNAsRNA, Long NoncodingapoptosisautophagyexosomeslncAY927529Prostate cancer

Identifiers

PMID34724861
PMCPMC8794529
OpenAlexW3210859692

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.