ArticleProceedings of the National Academy of Sciences of the United States of America1987
Synchrotron x-ray diffraction studies of actin structure during polymerization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A structural model of the profilin-formin pacemaker system for actin filament elongation.Scientific reports · 2022Article
- Direct observation of the molecular mechanism underlying protein polymerization.Science advances · 2022Article
- Single-molecule nanopore sensing of actin dynamics and drug binding.Chemical science · 2019Article
- A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways.Neural plasticity · 2019Review
- Bridging length scales to measure polymer assembly.Molecular biology of the cell · 2017Article
- Article
- Early nucleation events in the polymerization of actin, probed by time-resolved small-angle x-ray scattering.Scientific reports · 2016Article
- Difference in hydration structures between F-actin and myosin subfragment-1 detected by small-angle X-ray and neutron scattering.Biophysics (Nagoya-shi, Japan) · 2013Article
- Internal motions of actin characterized by quasielastic neutron scattering.European biophysics journal : EBJ · 2011Article
- Actin oligomers at the initial stage of polymerization induced by increasing temperature at low ionic strength: Study with small-angle X-ray scattering.Biophysics (Nagoya-shi, Japan) · 2010Article
- Tightly-bound divalent cation of actin.Journal of muscle research and cell motility · 1992Review
- The effect of organic cryosolvents on actin structure: studies by small angle X-ray scattering.European biophysics journal : EBJ · 1992Article
- Effects of lithium ions on actin polymerization in the presence of magnesium ions.The Biochemical journal · 1991Article
- An alternative pathway of actin filament elongation. The condensation of small oligomers.Journal of muscle research and cell motility · 1989Article
- Probing actin polymerization by intermolecular cross-linking.The Journal of cell biology · 1988Article
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3 authors.
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Abstract
Synchrotron x-ray diffraction was used to identify the oligomers that formed during the earliest stages of actin polymerization. Solution diffraction patterns from G-actin (monomer) and from F-actin (polymer) contain information about the size and shape of actin monomers and the length, width, and subunit organization of filaments. Comparison of patterns collected during polymerization reveals an increase in scatter at spacings greater than 9.0 nm; formation of scattering bands at 5.4,4.9, and 3.4 nm; formation of a scattering minimum at 6.5 nm; and the presence of an isosbestic point at 9.0 nm. These scattering bands arise from the formation of, and organization of subunits in, filaments. At various actin concentrations (0.37-5 mg/ml), the change in scatter in these regions follows simple exponential kinetics with no detectable lag. Our analysis of the x-ray patterns shows that by 0.4 sec after mixing, most of the actin has formed dimers, which then rapidly incorporate into oligomers. At 4 mg/ml the early oligomers increase in length to greater than 30.0 nm within 10 sec. These results suggest that under our conditions actin molecules condense into filaments without the rate-limiting formation of nuclei.
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