ArticlePlant and soil2021
Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF.
Article in Plant and soil, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Advances in root phenotyping: high-throughput imaging, computational tools, and integrative approaches for crop improvement.Journal of experimental botany · 2026Review
- X-ray micro-CT imaging to study foliar water uptake mechanisms in plants with contrasting leaf topography.The New phytologist · 2025Article
- Characterization of Root Hair Curling and Nodule Development in Soybean-Rhizobia Symbiosis.Sensors (Basel, Switzerland) · 2024Article
- Monte Carlo simulations of synchrotron X-ray dose affecting root growth during in vivo tomographic imaging.Scientific reports · 2023Article
- A method of cotton root segmentation based on edge devices.Frontiers in plant science · 2023Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsWe sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information.
methodsTo demonstrate the efficacy of the system we employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves.
resultsWith the application of contrast media we identify fluid flow in root vasculature and visualise anatomical features, which are otherwise often only observable in ex vivo microscopy, including: the xylem, metaxylem, pith, fibres in aerenchyma and leaf venation. We are also able to observe interactions between aerenchyma cross sectional area and solute transport in the root vasculature with depth.
conclusionsOur novel system was capable of successfully delivering sufficient contrast media into root and leaf tissues such that anatomical features could be visualised and internal fluid transport observed. We propose that our system could be used in future to study internal plant transport mechanisms and parameterise models for fluid flow in plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11104-020-04784-x.
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