ArticleHorticulture research2021
Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
- Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- EnhancedPlants (Basel, Switzerland) · 2025Article
- A split ribozyme system for in vivo plant RNA imaging and genetic engineering.Plant biotechnology journal · 2025Article
- Poplar transformation with variable explant sources to maximize transformation efficiency.Scientific reports · 2025Article
- Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.International journal of molecular sciences · 2024Review
- A small secreted protein serves as a plant-derived effector mediating symbiosis betweenHorticulture research · 2024Article
- Article
- Isolation of Haustorium Protoplasts Optimized by Orthogonal Design for Transient Gene Expression inPlants (Basel, Switzerland) · 2024Article
- A simple and efficient in planta transformation method based on the active regeneration capacity of plants.Plant communications · 2024Article
- Engineering Crassulacean Acid Metabolism in CCold Spring Harbor perspectives in biology · 2024Review
- A simple and efficient method for betalain quantification inFrontiers in plant science · 2024Article
- Developing a UV-visible reporter-assisted CRISPR/Cas9 gene editing system to alter flowering time in Chrysanthemum indicum.Plant biotechnology journal · 2023Article
- Efficient floral dip transformation method usingSaudi journal of biological sciences · 2023Article
- CRISPR/Cas9-based gene activation and base editing inHorticulture research · 2023Article
- Split selectable marker systems utilizing inteins facilitate gene stacking in plants.Communications biology · 2023Article
- Low-cost and automated phenotyping system "Phenomenon" for multi-sensor in situ monitoring in plant in vitro culture.Plant methods · 2023Article
- eYGFPuv-Assisted Transgenic Selection inPlants (Basel, Switzerland) · 2023Article
- Network of miR396-mRNA in Tissue Differentiation in Moso Bamboo (Plants (Basel, Switzerland) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Green fluorescent protein (GFP) has been widely used for monitoring gene expression and protein localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required for the visualization of GFP, limitings its application to fixed locations in samples. A reporter that can be visualized in real-time regardless the shape, size and location of the target samples will increase the flexibility and efficiency of research work. Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsis and tobacco) and two woody plant species (poplar and citrus). We observed bright fluorescence under UV light in all of the four plant species without any effects on plant growth or development. eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues. With a focus on in vitro transformation, we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identified visually during the callus stage and the shoot stage, enabling early and efficient selection of transformants. Furthermore, whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants. In addition, our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics. This work demonstrates the feasibility of utilizing 1x eYGFPuv in studies of gene expression and plant transformation in diverse plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.