Evidence map›Paper›PMID 34718789›Full record

ArticlePlant physiology2022

Dual expression and anatomy lines allow simultaneous visualization of gene expression and anatomy.

Britta M C Kümpers, Jingyi Han, John Vaughan-Hirsch, Nicholas Redman, Alexander Ware, Jonathan A Atkinson, Nicola Leftley, George Janes, Giuseppe Castiglione, Paul T Tarr and 4 more

Open access · hybridAbstract read
In one paragraph

Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Britta M C KümpersSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.ORCID 0000-0001-9931-6985
Jingyi HanSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.ORCID 0000-0003-1106-2461
John Vaughan-HirschSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Nicholas RedmanSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Alexander WareSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Jonathan A AtkinsonSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Nicola LeftleySchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
George JanesSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Giuseppe CastiglioneSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Paul T TarrHoward Hughes Medical Institute, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA.
Kevin PykeSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Ute VoßSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
Darren M WellsSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.ORCID 0000-0002-4246-4909
Anthony BishoppSchool of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.ORCID 0000-0003-2962-9542
University of Nottingham · GBCalifornia Institute of Technology · US

Funding

Biotechnology and Biological Sciences Research Council BB/L023555/1Biotechnology and Biological Sciences Research Council BB/M008770/1Biotechnology and Biological Sciences Research Council BB/M019837/1
6 · The paper itself

Abstract

Studying the developmental genetics of plant organs requires following gene expression in specific tissues. To facilitate this, we have developed dual expression anatomy lines, which incorporate a red plasma membrane marker alongside a fluorescent reporter for a gene of interest in the same vector. Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis (Arabidopsis thaliana) during lateral root organogenesis for a period of over 24 h. Coupled with the development of a flow cell and perfusion system, we follow changes in activity of the DII auxin sensor following application of auxin.

Indexed as

ArabidopsisCell MembraneGene Expression Regulation, PlantGenes, PlantGenes, ReporterPlant Growth RegulatorsPlant RootsUltrasonographyPlant Growth Regulators

Identifiers

PMID34718789
PMCPMC8774739
OpenAlexW3209096193

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.