ArticleBMC genomics2021
Maternal methionine supplementation during gestation alters alternative splicing and DNA methylation in bovine skeletal muscle.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Emerging opportunities for DNA methylation biomarkers in cattle improvement.The Journal of reproduction and development · 2026Review
- Developmental Programming and Postnatal Modulations of Muscle Development in Ruminants.Biology · 2025Review
- Maternal Overnutrition in Beef Cattle: Effects on Fetal Programming, Metabolic Health, and Postnatal Outcomes.Biology · 2025Review
- Impact of dietary supplementation of beef cows with rumen-protected methionine during the periconceptional period on prenatal growth and performance to weaning.Journal of animal science · 2025Article
- EnrichKit: a multi-omics tool for livestock research.Frontiers in genetics · 2025Article
- Article
- Unveiling the methionine cycle: a key metabolic signature and NR4A2 as a methionine-responsive oncogene in esophageal squamous cell carcinoma.Cell death and differentiation · 2024Article
- Methionine Supplementation Alleviates the Germ Cell Apoptosis Increased by Maternal Caffeine Intake in aNutrients · 2024Article
- Molecular and Cellular Mechanisms of Intramuscular Fat Development and Growth in Cattle.International journal of molecular sciences · 2024Review
- One-carbon metabolite supplementation increases vitamin B12, folate, and methionine cycle metabolites in beef heifers and fetuses in an energy dependent manner at day 63 of gestation.Journal of animal science · 2024Article
- Ado-Mediated Depletion of Taurine Impairs Mitochondrial Respiratory Capacity and Alters the Chromatin Landscape of Inguinal Adipose Tissue.Nutrients · 2023Article
- Maternal diet induces persistent DNA methylation changes in the muscle of beef calves.Scientific reports · 2023Article
- Differentially methylated regions identified in bovine embryos are not observed in adulthood.Animal reproduction · 2023Article
- DNA methylation may affect beef tenderness through signal transduction in Bos indicus.Epigenetics & chromatin · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe evaluation of alternative splicing, including differential isoform expression and differential exon usage, can provide some insights on the transcriptional changes that occur in response to environmental perturbations. Maternal nutrition is considered a major intrauterine regulator of fetal developmental programming. The objective of this study was to assess potential changes in splicing events in the longissimus dorsi muscle of beef calves gestated under control or methionine-rich diets. RNA sequencing and whole-genome bisulfite sequencing were used to evaluate muscle transcriptome and methylome, respectively.
resultsAlternative splicing patterns were significantly altered by maternal methionine supplementation. Most of the altered genes were directly implicated in muscle development, muscle physiology, ATP activities, RNA splicing and DNA methylation, among other functions. Interestingly, there was a significant association between DNA methylation and differential exon usage. Indeed, among the set of genes that showed differential exon usage, significant differences in methylation level were detected between significant and non-significant exons, and between contiguous and non-contiguous introns to significant exons.
conclusionsOverall, our findings provide evidence that a prenatal diet rich in methyl donors can significantly alter the offspring transcriptome, including changes in isoform expression and exon usage, and some of these changes are mediated by changes in DNA methylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.