ArticleEuropean journal of nutrition2022
Dietary bovine milk miRNAs transported in extracellular vesicles are partially stable during GI digestion, are bioavailable and reach target tissues but need a minimum dose to impact on gene expression.
Article in European journal of nutrition, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 93 citations in OpenAlex.
- Comparative small RNA profiling of donkey, human and bovine milk-derived extracellular vesicles reveals conserved and distinct miRNA abundance patterns.Food chemistry. Molecular sciences · 2026Article
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Cross-Species Analysis of Milk Extracellular Vesicles Reveals a Conserved Innate Core and a Divergent, Human-Specific Adaptive Immune Fraction.International journal of molecular sciences · 2026Article
- Conserved Core and Species-Specific Signatures in the Milk Exosomal microRNA Targetome: A Preliminary Comparative In Silico Analysis of Human, Cow, Goat and Donkey Milk.International journal of molecular sciences · 2026Article
- Extracellular vesicles in solid tumors: from tumor ecology to engineered therapeutics.Molecular cancer · 2026Review
- Recent Progress in Exosome-Derived Nanocarriers for Breast Cancer Therapy: Advances, Translational Barriers, and Scale-Up Considerations.Pharmaceutical research · 2026Review
- Therapeutic Effects of miR-21-5p-Enriched Milk Extracellular Vesicles on Alzheimer's Disease-Associated Neurotoxicity in Vitro.Cell biochemistry and biophysics · 2026Article
- Different approaches to assess extracellular vesicles from bovine colostrum.JDS communications · 2026Article
- A novel spray-dried milk extracellular vesicles formulation with long-term immunomodulatory activity and functional stability.Scientific reports · 2026Article
- Predominant miRNAs in Animal-Source Foods and Bioinformatic Analysis.Current issues in molecular biology · 2026Article
- Milk-Derived EVs from Different Animal Sources: An Overview on Their Detection, Isolation and Pleiotropic Exerted Effects.International journal of molecular sciences · 2026Review
- Impact of Holder Pasteurization on Extracellular Vesicles and Immunoregulatory MicroRNAs in Human Breast Milk.Journal of agricultural and food chemistry · 2026Article
- ncRNAs in breast milk of mothers with SLE: potential implications for neonatal immune development.Frontiers in immunology · 2026Review
- Milk-derived extracellular vesicles in cardiovascular therapy: emerging biological functions and therapeutic perspectives.Frontiers in bioengineering and biotechnology · 2026Review
- Isolation and Characterization of Extracellular Vesicles Derived from Mango Fruits.International journal of molecular sciences · 2025Article
- Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk.Metabolites · 2025Article
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Involvement of miRNAs in the Cluster of Metabolic Factors of MetS: Nutrition-Genome-MetS Axis.Journal of clinical medicine · 2025Review
- Isolation of bovine milk-derived extracellular vesicles via a capillary-channeled polymer (C-CP) fiber stationary phase.Analytical and bioanalytical chemistry · 2025Article
- Influence of Maternal Diet and Lactation Time on the Exosomal miRNA Cargo in Breast Milk.Foods (Basel, Switzerland) · 2025Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeExtracellular RNAs are unstable and rapidly degraded unless protected. Bovine-milk extracellular vesicles (EVs) confer protection to dietary miRNAs, although it remains unclear whether this importantly improves their chances of reaching host target cells to exert biological effects.
methodsCaco-2, HT-29, Hep-G2 and FHs-74 cell lines were exposed to natural/labelled milk EVs to evaluate cellular uptake. Five frequently reported human milk miRNAs (miR-146b-5p, miR-148a-3p, miR-30a-5p, miR-26a-5p, and miR-22-3p) were loaded into EVs. The intracellular concentration of each miRNA in cells was determined. In addition, an animal study giving an oral dose of loaded EVs in C57BL6/ mice were performed. Gene expression regulation was assessed by microarray analysis.
resultsDigestive stability analysis showed high overall degradation of exogenous miRNAs, although EV-protected miRNAs better resisted gastrointestinal digestion compared to free miRNAs (tenfold higher levels). Importantly, orally delivered EV-loaded miRNAs reached host organs, including brain, in mice. However, no biological effect has been identified.
conclusionMilk EVs protect miRNAs from degradation and facilitate cellular uptake. miRNA concentration in EVs from bovine milk might be insufficient to produce gene modulation. Nevertheless, sizable amounts of exogenous miRNAs may be loaded into EVs, and orally delivered EV-loaded miRNAs can reach tissues in vivo, increasing the possibility of exerting biological effects. Further investigation is justified as this could have an impact in the field of nutrition and health (i.e., infant formulas elaboration).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.