Evidence map›Paper›PMID 34715910›Full record

ArticleArthritis research & therapy2021

Immunoprofiling of early, untreated rheumatoid arthritis using mass cytometry reveals an activated basophil subset inversely linked to ACPA status.

H Koppejan, M Hameetman, G Beyrend, V van Unen, J C Kwekkeboom, A H van der Helm-van Mil, R E M Toes, F A van Gaalen

Open access · goldAbstract read
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In one paragraph

Article in Arthritis research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

H KoppejanDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands. h.j.koppejan@lumc.nl.ORCID 0000-0002-6046-9113
M HameetmanDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands.
G BeyrendDepartment of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
V van UnenDepartment of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
J C KwekkeboomDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands.
A H van der Helm-van MilDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands.
R E M ToesDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands.
F A van GaalenDepartment of Rheumatology, Leiden University Medical Center, PO box 9600 (Zone C1-R), Albinusdreef 2, 2233, ZA, Leiden, The Netherlands.
Leiden University Medical Center · NL

Funding

Stichting Vrienden van de Voormalige te Noordwijk gevestigde Reumakliniek Sole Mio NA
6 · The paper itself

Abstract

backgroundAutoantibody production is a hallmark of rheumatoid arthritis (RA). Anti-citrullinated protein antibodies (ACPA) are highly disease-specific, and their presence is associated with more severe disease and poor prognosis compared to ACPA-negative patients. However, the immune cell composition associated with antibody-positive/negative disease is incompletely defined. Mass cytometry (MC) is a high-dimensional technique offering new possibilities in the determination of the immune cell composition in rheumatic diseases. Here, we set up a broad phenotyping panel to study the immune cell profile of early untreated RA to investigate if specific immune cell subsets are associated with ACPA+ versus ACPA- RA.

methodsFreshly obtained PBMCs of early, untreated RA patients (8 ACPA+ and 7 ACPA-) were analysed using a 36-marker MC panel, including markers related to various immune lineages. Data were processed using Cytosplore for dimensional reduction (HSNE) and clustering. Groups were compared using Cytofast. A second validation cohort of cryopreserved PBMCs obtained from early RA patients (27 ACPA+ and 20 ACPA-) was used to confirm MC data by flow cytometry (FC). FC data were processed and analysed using both an unsupervised analysis pipeline and through manual gating.

resultsMC indicated no differences when comparing major immune lineages (i.e. monocytes, T and B cells), but highlighted two innate subsets: CD62L

conclusionsAlthough no differences in major lineages were found between early ACPA+ and ACPA- RA, this study identified the reduced presence of activated basophils in ACPA-negative disease as compared to ACPA-positive disease and thereby provides the first evidence for a connection between activated basophils and ACPA status.

Indexed as

Arthritis, RheumatoidBasophilsAnti-Citrullinated Protein AntibodiesB-LymphocytesCohort StudiesHumansAnti-Citrullinated Protein AntibodiesBasophilsCD62LImmune-profilingMass cytometryRheumatoid arthritis

Identifiers

PMID34715910
PMCPMC8555233
OpenAlexW3210460985

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.