Evidence map›Paper›PMID 34715587›Full record

ReviewCurrent opinion in chemical biology2021

Imaging organelle membranes in live cells at the nanoscale with lipid-based fluorescent probes.

N Dadina, J Tyson, S Zheng, L Lesiak, A Schepartz

Abstract readReview
In one paragraph

Review in Current opinion in chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023
    Article
  13. Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.Angewandte Chemie (International ed. in English) · 2023
    Article
  14. Lipid Expansion Microscopy.Journal of the American Chemical Society · 2022
    Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

N DadinaDepartment of Chemistry, University of California, Berkeley 94720, USA.
J TysonDepartment of Chemistry, University of California, Berkeley 94720, USA.
S ZhengDepartment of Chemistry, University of California, Berkeley 94720, USA.
L LesiakDepartment of Chemistry, University of California, Berkeley 94720, USA.
A SchepartzDepartment of Chemistry, University of California, Berkeley 94720, USA; Department of Molecular & Cell Biology, University of California, Berkeley 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley 94720, USA. Electronic address: schepartz@berkeley.edu.

Funding

Fluorescence tools that illuminate biology and inspire translationR35GM134963 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Alanna Schepartz · 2020 to 2026
$4.7M
Expanding the HIDE nanoscopy toolbox: More organelles, colors, and modalitiesR01GM131372 · NIGMS · YALE UNIVERSITY · PI SCHEPARTZ, ALANNA · 2019 to 2019
$299k
NIGMS NIH HHS R01 GM131372NIGMS NIH HHS R35 GM134963
6 · The paper itself

Abstract

Understanding how organelles interact, exchange materials, assemble, disassemble, and evolve as a function of space, time, and environment is an exciting area at the very forefront of chemical and cell biology. Here, we bring attention to recent progress in the design and application of lipid-based tools to visualize and interrogate organelles in live cells, especially at super resolution. We highlight strategies that rely on modification of natural lipids or lipid-like small molecules ex cellula, where organelle specificity is provided by the structure of the chemically modified lipid, or in cellula using cellular machinery, where an enzyme labels the lipid in situ. We also describe recent improvements to the chemistry upon which lipid probes rely, many of which have already begun to broaden the scope of biological questions that can be addressed by imaging organelle membranes at the nanoscale.

Indexed as

Fluorescent DyesOrganellesDiagnostic ImagingLipidsFluorescent DyesLipidsBioorthogonal chemistryFluorogenicFluorophoreLipidOrganelle InteractomeSuper-resolution microscopy

Identifiers

PMID34715587
PMCPMC9904808

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.