Quantification of intracellular HNO delivery with capillary zone electrophoresis.

Thilini N Amarakoon, Neng Ke, Craig A Aspinwall, Katrina M Miranda

Open access · greenAbstract read

In one paragraph

Article in Nitric oxide : biology and chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 48% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Thilini N AmarakoonDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA.

Neng KeDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA.

Craig A AspinwallDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA; BIO5 Institute, University of Arizona, Tucson, AZ, 85721, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, 85721, USA.

Katrina M MirandaDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA; BIO5 Institute, University of Arizona, Tucson, AZ, 85721, USA. Electronic address: kmiranda@email.arizona.edu.

University of Arizona · US

Funding

Stabilized Biomimetic Separation MediaR01GM095763 · NIGMS · UNIVERSITY OF ARIZONA · PI ASPINWALL, CRAIG A · 2011 to 2014

$1.1M

NIGMS NIH HHS R01 GM095763

6 · The paper itself

Abstract

Redox signaling, wherein reactive and diffusible small molecules are channeled into specific messenger functions, is a critical component of signal transduction. A central principle of redox signaling is that the redox modulators are produced in a highly controlled fashion to specifically modify biotargets. Thiols serve as primary mediators of redox signaling as a function of the rich variety of adducts, which allows initiation of distinct cellular effects. Coupling the inherent reactivity of thiols with highly sensitive and selective chemical analysis protocols can facilitate identification of redox signaling agents, both in solution and in cultured cells. Here, we describe use of capillary zone electrophoresis to both identify and quantify sulfinamides, which are specific markers of the reaction of thiols with nitroxyl (HNO), a putative biologically relevant reactive nitrogen species.

Indexed as

Cell Line, TumorElectrophoresis, CapillaryGlutathioneHumansNitrogen OxidesGlutathioneglutathione sulfinamideNitrogen OxidesnitroxylCapillary zone electrophoresisFluorescenceGlutathioneNitroxylSulfinamideThiol

Identifiers

PMID34715361

DOI10.1016/j.niox.2021.10.005

PMCPMC8758193

OpenAlexW3209863621

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.