ReviewFrontiers in genome editing2021
Protoplast Regeneration and Its Use in New Plant Breeding Technologies.
Review in Frontiers in genome editing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed.
- PEGylated Positively Charged Fluorescent Silica Nanoparticles for Enhanced Gene Delivery in Canola (ACS omega · 2026Article
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Toward in planta studies of persistent fungal viruses in a model plant.Virus research · 2026Article
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells.Journal of experimental botany · 2026Article
- Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (Biology · 2026Article
- Optimization of CRISPR/Cas9-mediated CtPDS knockout in guar protoplasts.The plant genome · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Aggregates-on-chip: a microfluidic device for crowding plant cells in culture.Frontiers in plant science · 2026Article
- Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering.Plant biotechnology journal · 2026Article
- Tiny but powerful: protoplast isolation inFrontiers in plant science · 2026Article
- Effect of Suberoylanilide Hydroxamic Acid and Phytosulfokine-Alpha on Successful Plant Regeneration from Embryogenic Callus-Derived Protoplasts of Garlic (International journal of molecular sciences · 2025Article
- Impedance flow cytometry for rapid quality assessment of protoplast cultures.Plant methods · 2025Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species.Scientific reports · 2025Article
- CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects.Plant molecular biology · 2025Review
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts - from General Tissue Engineering to Cellular Agriculture.Advanced biology · 2025Article
- Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal.Marine biotechnology (New York, N.Y.) · 2025Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of gene-editing technology holds tremendous potential for accelerating crop trait improvement to help us address the need to feed a growing global population. However, the delivery and access of gene-editing tools to the host genome and subsequent recovery of successfully edited plants form significant bottlenecks in the application of new plant breeding technologies. Moreover, the methods most suited to achieve a desired outcome vary substantially, depending on species' genotype and the targeted genetic changes. Hence, it is of importance to develop and improve multiple strategies for delivery and regeneration in order to be able to approach each application from various angles. The use of transient transformation and regeneration of plant protoplasts is one such strategy that carries unique advantages and challenges. Here, we will discuss the use of protoplast regeneration in the application of new plant breeding technologies and review pertinent literature on successful protoplast regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.