ArticleScientific reports2021
Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Direct Sampling Mass Spectrometry Analysis for the Assessment of Wounds: A Systematic Review.International wound journal · 2025Pooled it
- Safety and efficacy of topical melatonin add-on in healing of diabetic foot ulcer: a double blind, randomized clinical trial.BMC endocrine disorders · 2025Trial
- Lactate metabolism and protein lactylation in inflammatory and tumor microenvironments.Molecular biomedicine · 2026Review
- Fibroblast Dynamics Following Partial and Deep Burn Injury in a Reconstructed Human Skin Model.Tissue engineering and regenerative medicine · 2026Article
- Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin BJournal of animal science and biotechnology · 2025Article
- LASSO Logistic Regression for Predicting Postoperative Severe Pain After Hepatic Hemangioma Ablation.Journal of pain research · 2025Article
- Morphological and molecular preservation through universal preparation of fresh-frozen tissue samples for multimodal imaging workflows.Nature protocols · 2024Review
- Prediction of immune molecules activity during burn wound healing among elderly patients: in-silico analyses: experimental research.Annals of medicine and surgery (2012) · 2024Article
- The Preemptive Analgesic Effect of Capsaicin Involves Attenuations of Epidermal Keratinocytes Proliferation and Expression of Pro-Inflammatory Mediators After Plantar Incision in Rats.Journal of pain research · 2023Article
- Evaluation of Formalin-Fixed and FFPE Tissues for Spatially Resolved Metabolomics and Drug Distribution Studies.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Innate Immune System Response to Burn Damage-Focus on Cytokine Alteration.International journal of molecular sciences · 2022Review
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Authors and funding
12 authors.
Funding
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Abstract
Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin. LDH activity was visualised in injured and uninjured skin from 38 sub-acute burn patients. LDH activity aided the identification of spatially correlating immunocompetent cells in a sub-group of six patients. Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) was used to describe relative lactate and pyruvate abundance in burned and uninjured tissue. LDH activity was significantly increased in the middle and deep regions of burnt skin compared with superficial areas in burnt skin and uninjured tissue and positively correlated with post-burn time. Regions of increased LDH activity showed high pyruvate and low lactate abundance when examined with DESI-MSI. Areas of increased LDH activity exhibited cellular infiltration, including CD3 + and CD4 + T-lymphocytes and CD68 + macrophages. Our data demonstrate a steady increase in functional LDH activity in sub-acute burn wounds linked to cellular infiltration. The cell types associated are related to tissue restructuring and inflammation. This region in burn wounds is likely the focus of dysregulated inflammation and hypermetabolism.
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